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Cloud-clone

SKU(재고 관리 코드):PAA971Hu01

Polyclonal Antibody to Glypican 3 (GPC3)

Polyclonal Antibody to Glypican 3 (GPC3)

UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.

Product No.

PAA971Hu01

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA971Hu01-Recombinant Glypican 3 (GPC3)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; ICC; IHC-P; IHC-F; ELISA.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against GPC3. It has been selected for its ability to recognize GPC3 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Nature and Science Diagnostic Role of Serum Glypican-3 as a Tumor Marker for Hepatocellular Carcinoma Sciencepub:10043238
Journal of Applied Pharmaceutical Science Cytotoxic effects of antiglypican-3 against HepG2 cell line Ebscohost: Source
Asian Pacific Journal of Cancer Prevention Can Glypican3 be Diagnostic for Early Hepatocellular Carcinoma among Egyptian Patients? Pubmed: 24460300
Clin Biochem. Diagnostic accuracy of serum glypican-3 for hepatocellular carcinoma: A systematic review and meta-analysis Pubmed: 24362268
Science and Nature Diagnostic Role of Serum Glypican-3 as Hepatocellular Carcinoma Zolcat: Source
Alexandria Journal of Medicine Role of serum glypican-3 in the diagnosis and differentiation of small hepatocellular carcinoma from hepatitis-C virus cirrhosis ScienceDirect: S2090506814000062
J Egypt Natl Canc Inst. Comparing prothrombin induced by vitamin K absence-II (PIVKA-II) with the oncofetal proteins glypican-3, Alpha feto protein and carcinoembryonic antigen in diagnosing hepatocellular carcinoma among Egyptian patients. Pubmed:24841158
European Journal of Pharmacology Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma Pubmed:25449037
Hepatobiliary Pancreat Dis Int Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern abstract:abstract4525.shtml
Clinical and Molecular Hepatology  Glypican-3 level assessed by the enzyme-linked immunosorbent assay is inferior to alpha-fetoprotein level for hepatocellular carcinoma diagnosis pmc:articles
Wiley Glypican‐3: A promising biomarker for hepatocellular carcinoma diagnosis and treatment pubmed:28621802
Biomedicine & Pharmacotherapy Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and Pubmed:29669302
Journal of Cancer and Clinical Oncology Development and Validation of a Two-Site Immunoradiometric assay for Glypican-3 in plasma: Implication in Diagnosis of Hepatocellular Carcinoma Researchgate:Source
Turkish journal of oncology Serum Glypican-3, Vascular Endothelial Growth Factor, and Interleukin-6 Levels in Hepatocellular Carcinoma 10.5505:tjo.2018.1742
Intensive Care Medicine Experimental Elevated plasma glypicans are associated with organ failure in patients with infection Pubmed: 30618011
VALUE OF SERUM GLYPICAN 3 AS A PREDICTIVE TOOL FOR EARLY DIAGNOSIS OF HEPATOCELLULAR CARCINOMA (HCC)
ADVANCED RESEARCHES Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C
Life Sci Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and … Pubmed:35640776
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA971Hu02 Recombinant Glypican 3 (GPC3) Positive Control; Immunogen; SDS-PAGE; WB.
RPA971Hu01 Recombinant Glypican 3 (GPC3) Positive Control; Immunogen; SDS-PAGE; WB.
PAA971Hu01 Polyclonal Antibody to Glypican 3 (GPC3) WB; ICC; IHC-P; IHC-F; ELISA.
MAA971Hu21 Monoclonal Antibody to Glypican 3 (GPC3) WB; IHC; ICC; IP.
MAA971Hu22 Monoclonal Antibody to Glypican 3 (GPC3) WB; IHC; ICC; IP.
MAA971Hu24 Monoclonal Antibody to Glypican 3 (GPC3) WB; IHC; ICC; IP.
MAA971Hu23 Monoclonal Antibody to Glypican 3 (GPC3) WB; IHC; ICC; IP.
MAA971Hu25 Monoclonal Antibody to Glypican 3 (GPC3) WB; IHC; ICC; IP.
SEA971Hu ELISA Kit for Glypican 3 (GPC3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA971Hu Magnetic Luminex Assay Kit for Glypican 3 (GPC3) ,etc. Magnetic Luminex Assay for Antigen Detection.

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