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Cloud-clone

SKU(재고 관리 코드):PAB831Hu01

Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)

Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)

UOM
Concentration
Organism species
Western blotting: 1-5ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.

Product No.

PAB831Hu01

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB831Hu01-Recombinant Hedgehog Homolog, Sonic (SHH)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Mus musculus (Mouse)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml



SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Annals of the Rheumatic Diseases Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis pubmed:28495672
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB831Hu01 Recombinant Hedgehog Homolog, Sonic (SHH) Positive Control; Immunogen; SDS-PAGE; WB.
EPB831Hu61 Eukaryotic Hedgehog Homolog, Sonic (SHH) Positive Control; Immunogen; SDS-PAGE; WB.
APB831Hu01 Active Hedgehog Homolog, Sonic (SHH) Cell culture; Activity Assays.
APB831Hu61 Active Hedgehog Homolog, Sonic (SHH) Cell culture; Activity Assays.
PAB831Hu01 Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC; IP.
MAB831Hu22 Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB,IHC
MAB831Hu21 Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB,IHC
MAB831Hu24 Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC; IP.
RAB831Hu21 Recombinant Antibody to Hedgehog Homolog, Sonic (SHH) WB; IF; ICC; IHC; IP; FCM.
MAB831Hu23 Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC; IP.
SEB831Hu ELISA Kit for Hedgehog Homolog, Sonic (SHH) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB831Hu Mini Samples ELISA Kit for Hedgehog Homolog, Sonic (SHH) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB831Hu Magnetic Luminex Assay Kit for Hedgehog Homolog, Sonic (SHH) ,etc. Magnetic Luminex Assay for Antigen Detection.

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