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Cloud-clone

SKU(재고 관리 코드):PAA544Ca01

Polyclonal Antibody to Immunoglobulin G (IgG)

Polyclonal Antibody to Immunoglobulin G (IgG)

UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Product No.

PAA544Ca01

Organism Species

Canis familiaris; Canine (Dog) 

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

NPA544Ca01-Native Immunoglobulin G (IgG)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

n/a

Applications

WB

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IgG. It has been selected for its ability to recognize IgG in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Journal of Animal and Veterinary Advances Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine Medwell: 2010610616
Journal of Animal and Veterinary Advances Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus Medwell: 2010594600
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI Ijfans: Source
Poultry Science Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 Pubmed: 24570422
Pharmaceutical Development and Technology Foot and Mouth Disease virus-loaded fungal chitosan nanoparticles for intranasal administration: impact of formulation on physicochemical and immunological characteristics Pubmed: 23590209
BioMed Research International The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Hindawi:Source
Journal of Proteomics Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection Pubmed:24576640
Int J Occup Environ Health. Liver functions in silica-exposed workers in Egypt: possible role of matrix remodeling and immunological factors Pubmed:24999850
Food & Function Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice Pubmed:25308407
Oncology Reports Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer Pubmed:25529861
Biomed Res Int The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling PubMed: 26161394
Protein Pept Lett Stabilization of Human Immunoglobulin G Encapsulated within Biodegradable Poly (Cyclohexane-1, 4-diyl Acetone Dimethylene Ketal) (PCADK)/ Poly (Lactic-co-Glycolic Acid) (PLGA) Blend Microspheres PubMed: 26268563
Electrophoresis Growth promotion in pigs by oxytetracycline coincides with down reCavia (Guinea pig )lation of serum inflammatory parameters and of hibernation‐associated protein HP‐27 Pubmed:26914286
Neuropeptides Neuropeptide Y stimulates osteoblastic differentiation and VEGF expression of bone marrow mesenchymal stem cells related to canonical Wnt signaling activating in vitro. Pubmed:26707636
Archives of Biological Sciences Effect of oral administration of Lactobacillus paracasei L9 on mouse systemic immunity and the immune response in the intestine pid:46641600021Z
Asian J Pharm Clin Res INFLUENCE OF ZINCOVIT DROP (NUTRITIONAL FOOD SUPPLEMENT) ON IMMUNE SYSTEM IN NORMAL AND CYCLOPHOSPHAMIDE INTOXICATED WISTAR RATS journals:3790
journal of biotechnology Expression of α-1,6-fucosyltransferase (FUT8) in rice grain and immunogenicity evaluation of plant-specific glycans. pubmed:28013072
Sci Rep.  Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. pubmed:28220870
Toxicology Research Effect of dietary protein or calcium supplement on the expression of collagen I and dentine phosphoprotein of rats with dental fluorosis C6TX00288A
ntervirology Exposure of Piglets to Enteroviruses Investigated by an Immunoassay Based on the EV-G1 VP4 Peptide pubmed:27694750
Biomedicine & Pharmacotherapy Icariin promotes osteogenic differentiation of rat bone marrow stromal cells by activating the ERα-Wnt/β-catenin signaling pathway. pubmed:27764755
Food and Agricultural Immunology Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers 10.108:09540105.2017.1311308
Biomedicine & Pharmacotherapy In vitro and in vivo immunomodulatory potential of Pedicularis longiflora and Allium carolinianum in alloxan-induced diabetes in rats pubmed:29091887
Journal of Biochemical and Molecular Toxicology Fluoride or/and aluminum induced toxicity in guinea pig teeth with the low expression of dentine phosphoprotein 10.1002/jbt.21912
International Journal of Science and Research Impact of Silver Nanoparticles on Immune Responses as Vaccine against Activated Staphylococcus aureus Bacteria ISSN: 2319-7064
Molecular Medicine Reports Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. pubmed:28586019
Revista Brasileira de Zootecnia Influence of eggshell colour on egg yolk antibody level, incubation results, and growth in broiler breeders 10.1590/s1806-92902017000200004 
Water Research  Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model Pubmed:29655095
Clinical Immunology Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis Pubmed:30056130
Food and Agricultural Immunology Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers 10.1080:09540105.2018.1457013
Journal of Molecular Neuroscience A Novel scFv Anti-Aβ Antibody Reduces Pathological Impairments in APP/PS1 Transgenic Mice via Modulation of Inflammatory Cytokines and Aβ-related Enzymes Pubmed:30062438
Biological Trace Element Research Supplementation of Cr Methionine During Dry Period of Dairy Cows and Its Effect on Some Production and Biochemical Parameters During Early Lactation and on … Pubmed:29497999
Probiotics Antimicrob Proteins Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a … Pubmed:29305686
SAGE Open Medicine Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes Pubmed:29623201
Journal of Biological Chemistry Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids Pubmed: 30237167
ACS Applied Materials & Interfaces Fabrication and characterization of core-shell nanofibers using a next-generation airbrush for biomedical applications Pubmed: 30433758
Molecular Human Reproduction Phenotypic variations in transferred progeny due to genotype of surrogate mother: Surrogate mother modulates transferred progeny Pubmed: 30445548
Bioscience, Biotechnology, and Biochemistry In vitro and in vivo cancer cell apoptosis triggered by competitive binding of Cinchona alkaloids to the RING domain of TRAF6
Journal of proteome research Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection
International Immunopharmacology Ginsenoside Rg3 improves cyclophosphamide-induced immunocompetence in Balb/c mice Pubmed: 30974284
Utjecaj dodatka selena u prihrani na hematološku sliku i biokemijske pokazatelje u jelena lopatara (Dama dama L.)
Biogerontology Age-related chemokine alterations affect IgA secretion and gut immunity in female mice Pubmed: 32277312
International Journal of Biological Macromolecules Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickens Pubmed: 32224178
Pharmaceutics Efficacy of Local Minocycline Agents in Treating Peri-Implantitis: An Experimental In Vivo Study in Beagle Dogs Pubmed: 33114168
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Physicochemical characterization and immunomodulatory activity of sulfated Chinese yam polysaccharide Pubmed: 33010421
Pharmaceutics Locally Applied Slow-Release of Minocycline Microspheres in the Treatment of Peri-Implant Mucositis: An Experimental In Vivo Study Pubmed: 32708741
BIOLOGICAL TRACE ELEMENT RESEARCH Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle Pubmed: 32583225
Molecular Medicine Reports Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism Pubmed: 33000198
South African Journal of Botany Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo
Journal of Functional Foods Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts
Italian Journal of Animal Science Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the …
Regenerative Biomaterials A comparative study of autogenous, allograft and artificial bone substitutes on bone regeneration and immunotoxicity in rat femur defect model
ACS APPLIED BIO MATERIALS Curcumin loaded nanostructure hybrid lipid capsules for co-eradication of breast cancer and cancer stem cells with enhanced anticancer efficacy
博士论文 Women Scientist Scientist Mentor
sensors and actuators b-chemical A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate
THERANOSTICS Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without?¡­ 33456566
Life Sci Celecoxib ameliorates liver cirrhosis via reducing inflammation and oxidative stress along spleen-liver axis in rats 33577848
Animals (Basel) Handling Associated with Drenching Does Not Impact Survival and General Health of Low Birth Weight Piglets. Animals 2021, 11, 404 33562568
Environ Pollut Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure 34090073
Veterinary Microbiology Optimized GAPDH-truncated immunogen of Streptococcus equi elicits an enhanced immune response and provides effective protection in a mouse model 33647714
Experimental and Therapeutic Medicine Insulin-like growth factor-1 improves postoperative cognitive dysfunction following splenectomy in aged rats 33574912
British Journal of Pharmacology The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus
Journal of Chinese Pharmaceutical Sciences A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle
Neonatal Buza??larda ?ki Farkl? ?mmunomod¨¹lat?r¨¹n Buza?? ?shalleri ¨¹zerine Etkileri
Microbiology Spectrum Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows 34494854
Pediatrics International Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus 34559910
Toxicology Protection from Benzene-induced Immune Dysfunction in Mice Pubmed:35090963
Food Funct Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components Pubmed:35438093
Cell Death & Disease Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death Pubmed:35732625
Nature Communications Integrative epigenomic and transcriptomic analysis reveals the requirement of JUNB for hematopoietic fate induction Pubmed:35668082
Food and Agricultural Immunology The effect of the multi-strain probiotic preparation EM Bokashi® on selected parameters of the cellular immune response in pigs
陆军军医大学学报 信筒子醌对系统性红斑狼疮小鼠免疫调节和治疗作用
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
NPA544Ca01 Native Immunoglobulin G (IgG) Positive Control; Immunogen; SDS-PAGE; WB.
PAA544Ca01 Polyclonal Antibody to Immunoglobulin G (IgG) WB
LAA544Ca71 Biotin-Linked Polyclonal Antibody to Immunoglobulin G (IgG) WB; IHC; ICC.
LAA544Ca91 HRP-Linked Polyclonal Antibody to Immunoglobulin G (IgG) WB; IHC; ICC.
SAA544Ca09 HRP-Linked Rabbit Anti-Canine IgG Polyclonal Antibody WB; IHC; ICC.
SAA544Ca07 Biotin-Linked Rabbit Anti-Canine IgG Polyclonal Antibody WB; IHC; ICC.
SAA544Ca08 FITC-Linked Rabbit Anti-Canine IgG Polyclonal Antibody WB; IHC; ICC; IF.
CEA544Ca ELISA Kit for Immunoglobulin G (IgG) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA544Ca CLIA Kit for Immunoglobulin G (IgG) Chemiluminescent immunoassay for Antigen Detection.
LMA544Ca Magnetic Luminex Assay Kit for Immunoglobulin G (IgG) ,etc. Magnetic Luminex Assay for Antigen Detection.
KSA544Ca11 ELISA Kit DIY Materials for Immunoglobulin G (IgG) Main materials for "Do It (ELISA Kit) Yourself".

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