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Cloud-clone

SKU(재고 관리 코드):PAB547Hu01

Polyclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO)

Polyclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO)

UOM
Concentration
Organism species
Western blotting: 1-5ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.

Product No.

PAB547Hu01

Organism Species

Homo sapiens (Human) 

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB547Hu01-Recombinant Indoleamine-2,3-Dioxygenase (IDO)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml



SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IDO. It has been selected for its ability to recognize IDO in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Blood CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects Pubmed: 21803856
Clinical Transplantation Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT Wiley: source
Indonesian Journal of Cancer Chemoprevention Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell Iregway: Source
PLoS ONE CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms PubMed: PMC3683035
Hepatology Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma Pubmed: 23960017
Free Radical Biology and Medicine Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans ScienceDirect: S0891584913006345
Frontiers in Oncology Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation Frontiersin:Source
Tissue Engineering Part A Immunogenicity and escape mechanisms of allogeneic tendon-derived stem cells Pubmed:24813640
Immunobiology. The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered Pubmed:24947894
Apoptosis Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes Pubmed:25577277
Front Oncol Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation PubMed: 26029659
Acta Universitatis Tamperensis Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties Handle: 10024
PLoS One Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase pubmed:27695127
Journal of Biomedical Nanotechnology Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility Pubmed:29883557
Biochemistry Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells
American Journal of Pathology Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD Mice Pubmed: 30980800
international immunopharmacology Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression Pubmed: 31176083
Stem Cell Reports Richard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4
International Journal of?Tryptophan?Research Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡­ 33343199
Stem Cell Reports Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4
Transl Psychiatry Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … 34802039
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB547Hu01 Recombinant Indoleamine-2,3-Dioxygenase (IDO) Positive Control; Immunogen; SDS-PAGE; WB.
APB547Hu01 Active Indoleamine-2,3-Dioxygenase (IDO) Cell culture; Activity Assays.
PAB547Hu01 Polyclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO) WB; IHC; ICC; IP.
LAB547Hu71 Biotin-Linked Polyclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO) WB; IHC; ICC.
LAB547Hu81 FITC-Linked Polyclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO) WB; IHC; ICC; IF.
MAB547Hu22 Monoclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO) WB; IHC; ICC; IP.
MAB547Hu21 Monoclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO) WB; IHC; ICC; IP.
MAB547Hu23 Monoclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO) WB; IHC; ICC; IP.
LAB547Hu72 Biotin-Linked Monoclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO) WB; IHC; ICC.
SEB547Hu ELISA Kit for Indoleamine-2,3-Dioxygenase (IDO) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB547Hu Magnetic Luminex Assay Kit for Indoleamine-2,3-Dioxygenase (IDO) ,etc. Magnetic Luminex Assay for Antigen Detection.
PSB547Hu01 Antibody Pair for Indoleamine-2,3-Dioxygenase (IDO) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB547Hu01 ELISA Kit DIY Materials for Indoleamine-2,3-Dioxygenase (IDO) Main materials for "Do It (ELISA Kit) Yourself".

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