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SKU(재고 관리 코드):PAA033Bo01

Polyclonal Antibody to Interferon Alpha (IFNa)

Polyclonal Antibody to Interferon Alpha (IFNa)

UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Product No.

PAA033Bo01

Organism Species

Bos taurus; Bovine (Cattle).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA033Bo01-Recombinant Interferon Alpha (IFNa)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IFNa. It has been selected for its ability to recognize IFNa in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Journal of Animal and Veterinary Advances Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine Medwell: 2010610616
Journal of Animal and Veterinary Advances Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus Medwell: 2010594600
Ann Rheum Dis. The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats PubMed: 23172753
Nanomedicine Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform PubMed: 23394132
International journal of experimental pathology Experimental rat model for alcohol-induced osteonecrosis of the femoral head PubMed: PMC3781777
Antiviral Research A recombinant adenovirus bicistronically expressing porcine interferon-α and interferon-γ enhances antiviral effects against foot-and-mouth disease virus ScienceDirect: S0166354214000266
British Journal of Pharmacology Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells Onlinelibrary: bph.12440
BMC Cancer High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model Pubmed:24950740
PLoS One. Respiratory Mechanics and Plasma Levels of Tumor Necrosis Factor Alpha and Interleukin 6 Are Affected by Gas Humidification during Mechanical Ventilation in Dogs Pubmed:25036811
Veterinary Research Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain Pubmed:25106750
Asian Pac J Trop Med Effect of SecinH3 on lung injury induced by sepsis of rats PubMed: 26706678
PLoS One siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro Pubmed:26886455
Plos One Sulforaphane Suppresses Hepatitis C Virus Replication by Up-ReCavia (Guinea pig )lating Heme Oxygenase-1 Expressionthrough PI3K/Nrf2 Pathway. Pubmed:27023634
PLoS One. Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in Cavia (Guinea pig )inea pigs. Pubmed:23776655
Veterinary microbiology Impact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain). pubmed:27599928
International Journal of Clinical and Experimental Pathology Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells files:ijcep0036479.pdf
Scandinavian Journal of Immunology Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress. pubmed:27384769
Saudi Journal of Gastroenterology Deoxyschizandrin suppresses dss-induced ulcerative colitis in mice. pubmed:27976641
Biochemical and Biophysical Research Communications MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B pubmed:28342861
Virology The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection pubmed:28988058
Antiviral Research Coinjection of a vaccine and anti-viral agents can provide fast-acting protection from foot-and-mouth disease pubmed:28454913
Molecular Therapy. Methods & Clinical Development Anti-high mobility group box 1 antibody ameliorates albuminuria in MRL/lprlupus-prone mice pubmed:28649578
Journal of Virology Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation. pubmed:28915824
Clinical Immunology Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis Pubmed:30056130
International forum of allergy & rhinology Role of Toll‐like receptor 9 signaling on activation of nasal polyp–derived fibroblasts and its association with nasal polypogenesis Pubmed:29901280
LUPUS Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus Pubmed: 30336754
Journal of Pineal Research Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice Pubmed: 30929267
Veterinary Immunology and Immunopathology Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens
international immunopharmacology Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice Pubmed: 31228813
FASEB Journal Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1
Transboundary and Emerging Diseases Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in China Pubmed: 32187856
archives of virology A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in piglets Pubmed: 32253616
Autophagy LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection Pubmed: 32924761
THERIOGENOLOGY Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasion Pubmed: 32810792
Int Immunopharmacol BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice Pubmed: 32717566
Molecular Medicine Reports Aquaporin‑4 deletion ameliorates enterovirus 71 infection in mice Pubmed: 32582978
Virulence Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon …
РОССИЙСКАЯ СЕЛЬСКОХОЗЯЙСТВЕННАЯ НАУКА ГОРМОНАЛЬНЫЙ И ЦИТОКИНОВЫЙ ПРОФИЛЬ КРОВИ МОЛОЧНЫХ КОРОВ В РАННИЙ ПЕРИОД ГЕСТАЦИИ
Communications Biology Influenza virus neuraminidase regulates host CD8+ T-cell response in mice 33293641
Animals Concentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants 33494350
Int J Dermatol IFN©\¦Á and TNF©\¦Á serum levels and their association with disease severity in Egyptian children and adults with alopecia areata 34008204
Dig Dis Sci Orexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway 34097168
Clinical findings of Talaromyces marneffei infection among patients with anti-interferon-¦Ã immunodeficiency: a prospective cohort study 34144700
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats 34555716
Carbohydrate Polymers The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides 34823813
Poult Sci Effects of different selenium sources on duodenum and jejunum tight junction network and growth performance of broilers in a model of fluorine-induced … Pubmed:35066382
Veterinary Microbiology Self-assembled BPIV3 nanoparticles can induce comprehensive immune responses and protection against BPIV3 challenge by inducing dendritic cell maturation in … Pubmed:35395543
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
EPA033Bo61 Eukaryotic Interferon Alpha (IFNa) Positive Control; Immunogen; SDS-PAGE; WB.
RPA033Bo01 Recombinant Interferon Alpha (IFNa) Positive Control; Immunogen; SDS-PAGE; WB.
PAA033Bo01 Polyclonal Antibody to Interferon Alpha (IFNa) WB; IHC; ICC; IP.
MAA033Bo21 Monoclonal Antibody to Interferon Alpha (IFNa) WB; IHC; ICC; IP.
SEA033Bo ELISA Kit for Interferon Alpha (IFNa) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA033Bo Instant ELISA Kit for Interferon Alpha (IFNa) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA033Bo CLIA Kit for Interferon Alpha (IFNa) Chemiluminescent immunoassay for Antigen Detection.
LMA033Bo Magnetic Luminex Assay Kit for Interferon Alpha (IFNa) ,etc. Magnetic Luminex Assay for Antigen Detection.

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