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Cloud-clone
SKU(재고 관리 코드):PAA222Eq01
Polyclonal Antibody to Interferon Beta (IFNb)
Polyclonal Antibody to Interferon Beta (IFNb)
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA222Eq01
Organism Species
Equus caballus; Equine (Horse).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against IFNb. It has been selected for its ability to recognize IFNb in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| 20 | Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathway PubMed: 21355876 |
| Journal of General Virology | Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3. Sgm: 9182080 |
| Biochemical and Biophysical Research Communications | Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damage ScienceDirect: S0006291X09019822 |
| PLoS One | Intact Type I Interferon Production and IRF7 Function in Sooty Mangabeys PubMed: PMC3757038 |
| PLoS Neglected Tropical Diseases | Innate Immune Response to Rift Valley Fever Virus in Goats PubMed: PMC3335883 |
| BioMed Research International | Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages Pubmed: 24288685 |
| Vet Microbiol. | An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages. Pubmed: 23850441 |
| Food and Chemical Toxicology | Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in mice Pubmed: 23535186 |
| Veterinary immunology and immunopathology | Experimental model of equine alveolar macrophage stimulation with TLR ligands Pubmed: 23815824 |
| American journal of respiratory cell and molecular biology | Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages Pubmed: 24502337 |
| FEBS Lett. | Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunity Pubmed:24530528 |
| Veterinary Research | Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain Pubmed:25106750 |
| PLOS Neglected Tropical Diseases | MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production Pubmed:25122182 |
| Veterinary Research | The innate immune response of equine bronchial epithelial cells is altered by training Pubmed:25595212 |
| molecular and celluar biology | SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity PubMed: 25939384 |
| Arch Virol | Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virus PubMed: 25797196 |
| J | Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic Cells PubMed: 26258404 |
| Virus | PKR activation enhances replication of classical swine fever virus in PK-15 cells PubMed: 25899421 |
| PLoS One | siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro Pubmed:26886455 |
| Scientific Reports | Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4 Pubmed:27090571 |
| Front Microbiol | Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo Pubmed:27252695 |
| Canadian Journal of Physiology and Pharmacology | Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25. pubmed:28134560 |
| International Journal of Molecular Medicine | Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function pubmed:27176664 |
| International Journal of Clinical and Experimental Pathology | Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells files:ijcep0036479.pdf |
| Poultry Science | ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages pubmed:27486255 |
| Journal of Virology | Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed: |
| Biochemical and Biophysical Research Communications | MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B pubmed:28342861 |
| Virology | The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection pubmed:28988058 |
| EMBO Journal | Zika virus elicits inflammation to evade antiviral response by cleaving cGAS via NS1‐caspase‐1 axis Pubmed:30065070 |
| Toxicology and applied pharmacology | Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult mice Pubmed:30017639 |
| Archives of Virology | MAVS induces a host cell defense to inhibit CSFV infection Pubmed:29556776 |
| Journal of virology | Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activation Pubmed: 30518647 |
| Veterinary Immunology and Immunopathology | Molecular cloning and functional characterization of porcine 2′, 5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory?… Doi: 10.1016/j.vetimm.2019.01.003 |
| Science Signaling | HIPK2 is necessary for type I interferon–mediated antiviral immunity Doi: 10.1126/scisignal.aau4604 |
| INTERNATIONAL MEDICAL RESEARCH | Evaluation of serum interferons in patients with age-related macular degeneration Pubmed: 31007694 |
| Veterinary Immunology and Immunopathology | Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens |
| International journal of biological macromolecules | Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction Pubmed: 31284005 |
| Journal of virology | Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB Activation Pubmed: 31243133 |
| The Journal of Latvian Academy of Sciences | Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia |
| Cancer Medicine | The immunostimulatory effects and pro‐apoptotic activity of rhCNB against Lewis lung cancer is mediated by Toll‐like receptor 4 Pubmed: 31218844 |
| FASEB Journal | Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1 |
| PLOS Pathogens | Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion Pubmed: 31539404 |
| Autophagy | Induction of autophagy and suppression of type I IFN secretion by CSFV Pubmed: 32160078 |
| MOLECULAR IMMUNOLOGY | BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activation Pubmed: 33053462 |
| Autophagy | LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection Pubmed: 32924761 |
| Arch Virol . | Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway Pubmed: 32532134 |
| Virulence | Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon … |
| bioRxiv | Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance |
| Res Vet Sci | Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells 34416463 |
| Eur J Immunol | Anti‐IFN‐α/‐ω neutralizing antibodies from COVID‐19 patients correlate with downregulation of IFN response and laboratory biomarkers of disease severity Pubmed:35419822 |
| ResearchSquare | Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I |
| Catalog No. | Related products for research use of Equus caballus; Equine (Horse) Organism species | Applications (RESEARCH USE ONLY!) |
| APA222Eq01 | Active Interferon Beta (IFNb) | Cell culture; Activity Assays. |
| RPA222Eq01 | Recombinant Interferon Beta (IFNb) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA222Eq01 | Polyclonal Antibody to Interferon Beta (IFNb) | WB; IHC; ICC; IP. |
| MAA222Eq21 | Monoclonal Antibody to Interferon Beta (IFNb) | WB; IHC; ICC; IP. |
| SEA222Eq | ELISA Kit for Interferon Beta (IFNb) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA222Eq | Magnetic Luminex Assay Kit for Interferon Beta (IFNb) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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