Cloud-clone
SKU(재고 관리 코드):PAC008Hu01
Polyclonal Antibody to Interleukin 35 (IL35)
Polyclonal Antibody to Interleukin 35 (IL35)
Western blotting: 0.5-3µg/mL;
;Immunohistochemistry: 5-30µg/mL;
;Immunocytochemistry: 5-30µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
PAC008Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPC008Hu01-Recombinant Interleukin 35 (IL35)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against IL35. It has been selected for its ability to recognize IL35 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Oncology Letters |
Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia. PubMed: 22783403 |
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Journal of Maternal-Fetal and Neonatal Medicine |
Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease Pubmed: 24175856 |
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Journal of Reproductive Immunology |
What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? Pubmed: 24368037 |
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Human Immunology |
The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia ScienceDirect: S0198885913005776 |
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Folia Biologica (Praha) |
Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers Pubmed:24785110 |
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Molecular Medicine Reports |
Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Pubmed:25323532 |
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Rom J Morphol Embryol. |
The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy Pubmed:25611266 |
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Journal of Immunotherapy |
Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity PubMed: 25962106 |
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PLOS ONE |
Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis PubMed: 26204444 |
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Rheumatology (Oxford) |
Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease PubMed: 26231346 |
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Cytokine |
Anti-inflammatory effects of interleukin-35 in acquired aplastic anemia PubMed: 26282938 |
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J Biol Chem |
Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway PubMed: 26085094 |
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Br J Haematol |
Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia PubMed: 25640666 |
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Autoimmunity Reviews |
New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literature Pubmed:26876383 |
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Clinical research |
Increased plasma concentrations of interleukin 35 in patients with coronary artery disease Journal:-19 |
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Clinical Rheumatology |
The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis pubmed:27502600 |
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Respiratory Medicine |
Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis pubmed:27492538 |
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International Journal of Molecular Medicine |
Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines. pubmed:27460435 |
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上海口腔医学 |
IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析 QK:98604X |
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Reproductive Sciences |
Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferation pubmed:28659009 |
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EXPERIMENTAL AND THERAPEUTIC MEDICINE |
IL‑35 may maintain homeostasis of the immune microenvironment in periodontitis issn:2542-3975 |
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The Journal of Rheumatology |
Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritis pubmed:29247146 |
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Cellular & Molecular Immunology |
Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro pubmed:28713164 |
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Molecular Medicine Reports |
Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. pubmed:29257310 |
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Asian pacific journal of allergy and immunology |
Evaluation of serum interleukin- 35 level in children with persistent asthma. pubmed:27996283 |
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PLoS One |
Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio pubmed:29236782 |
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Hepatology Communications |
Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation 10.1002/hep4.1052 |
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International Journal of Clinical and Experimental Medicine |
Clinical significance of IL-35 expression in the progression of clear cell renal cell carcinoma ISSN:1940-5901 |
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OncoTargets and Therapy |
Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy. pubmed:28761357 |
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Annals of Rheumatic Diseases |
AB0734 Serum il-35 levels in systemic sclerosis and relationship with clinical features 10.1136:annrheumdis-2018-eular.2605 |
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Tohoku Journal of Experimental Medicine |
Interleukin-35 as a New Biomarker of Renal Involvement in Lupus Nephritis Patients Pubmed:29576585 |
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Turkish Journal Of Medical Sciences |
The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility Pubmed:29914245 |
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Oncotarget |
Pro-tumoral immune cell alterations in wild type and Shb-deficient mice in response to 4T1 breast carcinomas Pubmed:29721156 |
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Journal of Clinical Rheumatology |
Serum Interleukin 35 Levels in Systemic Sclerosis and Relationship With Clinical Features. Pubmed: 30431486 |
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Oncology Letters |
Interleukin‑35 is associated with the tumorigenesis and progression of prostate cancer |
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Journal of Clinical Investigation |
Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35 Pubmed: 31264967 |
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Cells |
CD4+ FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired Pubmed: 32111105 |
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Cytokine |
Interleukin-35 in idiopathic inflammatory myopathies Pubmed: 33128920 |
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FEBS Open Bio |
IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1 Pubmed: 33064893 |
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Cancer Cell International |
Interleukin-35 promotes progression of prostate cancer and inhibits anti-tumour immunity Pubmed: 33041668 |
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Journal of Neuroinflammation |
Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain Pubmed: 32375811 |
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Int Immunopharmacol |
Association of IL-35 expression and gene polymorphisms in rheumatoid arthritis 33307515 |
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Archives of Medical Science |
Decreased interleukin-35 levels and CD4+ EBI3+ T cells in patients with type 1 diabetes and the effects of the antibody against CD20 (rituximab) 33488880 |
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Asia Pac Allergy |
Anti-inflammatory roles of interleukin-35 in the pathogenesis of Japanese cedar pollinosis 34386410 |
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Int Immunopharmacol |
Pentosan Polysulfate Sodium augments the therapeutic effect of 5-Aminosalicylic Acid in DSS colitis model; the role of IL-35 expression Pubmed:35247859 |
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Biology and Medical Researches |
Association of three single nucleotide polymorphisms of interleukin-35 for detection and prediction of women with breast cancer in Erbil city |
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Research Square |
Analysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoid |
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Catalog No. |
Related products for research use of Homo sapiens (Human) Organism species |
Applications (RESEARCH USE ONLY!) |
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LMC008Hu |
Magnetic Luminex Assay for Antigen Detection. |
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PSC008Hu01 |
ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
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KSC008Hu01 |
Main materials for "Do It (ELISA Kit) Yourself". |
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