Cloud-clone
SKU(재고 관리 코드):PAH839Hu01
Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)
Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1)
Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
PAH839Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPH839Hu01-Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Mus musculus (Mouse), Sus scrofa; Porcine (Pig)
Applications
WB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against IDH1. It has been selected for its ability to recognize IDH1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Molecular & Cellular Proteomics |
Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic Analysis Mcponline: 008821 |
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Molecular & Cellular Proteomics |
Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic Analysis Pubmed: 22064513 |
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Clinical Cancer Research |
Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancer Pubmed: 24046070 |
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Onco Targets Ther |
The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cells PubMed: 25914546 |
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Oncogene |
Metformin sensitizes endometrial cancer cells to chemotherapy through IDH1-induced Nrf2 expression via an epigenetic mechanism Pubmed:29921847 |
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Molecular Oncology |
IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancer Pubmed:29465809 |
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Cancers |
Serological Biomarkers for Early Detection of Hepatocellular Carcinoma: A Focus on Autoantibodies against Tumor-Associated Antigens Encoded by Cancer Driver … Pubmed: 32443439 |
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International Journal of Cancer |
Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancer Pubmed:35689436 |
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