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Cloud-clone

SKU(재고 관리 코드):PAA698Mu01

Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)

Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)

UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.

Product No.

PAA698Mu01

Organism Species

Mus musculus (Mouse).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA698Mu01-Recombinant Macrophage Migration Inhibitory Factor (MIF)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Homo sapiens (Human), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MIF. It has been selected for its ability to recognize MIF in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Kidney International Targeted reduction of advanced glycation improves renal function in obesity PubMed: 21412218
The Journal of Immunology Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the Gut Jimmunol: 5673
The Journal of Endocrinology Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver disease PubMed: PMC3757527
The Journal of Immunology Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing mice PubMed: PMC3466372
Diabetologia Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes Pubmed:24957662
BioMed Research International The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder PubMed: 25705692
Diabetologia Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease. pubmed:28160092
Life Sciences Protective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis pubmed:28919396
Scientific Reports Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic Stroke Pubmed:29335619
Ocular Immunology and Inflammation Immune Response and Mechanisms of IFN-γ in Administration for Keratomycosis Pubmed: 30307777
International Journal of Immunopathology and Pharmacology Effect of voluntary running on expression of myokines in brains of rats with depression Pubmed: 30834799
Biomarkers in Heart Failure and Associated Diseases Biomarkers in Heart Failure and Associated Diseases
Science China-Life Sciences Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation Pubmed: 31209799
Folia Morphologica Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expression Pubmed: 31802473
Biomed Pharmacother Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis Pubmed: 32062385
Sci Rep Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population 33850223
Commun Biol Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes Pubmed:35115654
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APA698Mu01 Active Macrophage Migration Inhibitory Factor (MIF) Cell culture; Activity Assays.
RPA698Mu02 Recombinant Macrophage Migration Inhibitory Factor (MIF) Positive Control; Immunogen; SDS-PAGE; WB.
RPA698Mu01 Recombinant Macrophage Migration Inhibitory Factor (MIF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA698Mu01 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
PAA698Mu02 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) WB; IHC; ICC; IP.
SEA698Mu ELISA Kit for Macrophage Migration Inhibitory Factor (MIF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA698Mu CLIA Kit for Macrophage Migration Inhibitory Factor (MIF) Chemiluminescent immunoassay for Antigen Detection.
LMA698Mu Magnetic Luminex Assay Kit for Macrophage Migration Inhibitory Factor (MIF) ,etc. Magnetic Luminex Assay for Antigen Detection.

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