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SKU(재고 관리 코드):PAA099Mu02

Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13)

Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13)

UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Product No.

PAA099Mu02

Organism Species

Mus musculus (Mouse).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA099Mu02-Recombinant Matrix Metalloproteinase 13 (MMP13)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Homo sapiens (Human), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MMP13. It has been selected for its ability to recognize MMP13 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression ScienceDirect: S0925443911001323

Journal of Physiology and Biochemistry

Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats SpringerLink: h875w73171w2147w

FEBS Journal

Adenosine A2a Receptor Activation and Hyaluronan Fragment Inhibition Reduce Inflammation in Mouse Articular Chondrocytes Stimulated with Il-1beta Wiley: source

Arthritis & Rheumatism

Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice Wiley: source

Gene

Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors ScienceDirect: S0378111911007347

PLoS ONE

A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source

Arthritis Rheumatol

Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression. Pubmed: 24578232

Arthritis & Rheumatology

Identification of Alpha 2 Macroglobulin (A2M) as a master inhibitor of cartilage degrading factors that attenuates post-traumatic osteoarthritis progression Onlinelibrary: art.38576

Thromb Haemost.

Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655

Arthritis & Rheumatol

Identification of α2-Macroglobulin as a Master Inhibitor of Cartilage-Degrading Factors That Attenuates the Progression of Posttraumatic Osteoarthritis Pubmed:24578232

Inflammation.?

Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261

Cellular Physiology and Biochemistry

Inverse Regulation of Early and Late Chondrogenic Differentiation by Oxygen Tension Provides Cues for Stem Cell-Based Cartilage Tissue Engineering Pubmed:25632940

Osteoarthritis and Cartilage

Depletion of gangliosides enhances cartilage degradation in mice Sciencedirect:S1063458413010364

Biol Res

The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis PubMed: 26578310

PLoS One

Interleukin-1 Receptor Antagonist Has a Novel Function in the Regulation of Matrix Metalloproteinase-13 Expression PubMed: 26474296

Cell Mol Immunol 

Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496

Scientific Reports

Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds pubmed:27821864

International Journal of Contemporary Medical Research

Matrix Metalloproteinase-13 (MMP-13): A Novel Tumor Marker for Diagnosis of Breast Carcinoma uploads:7

Scientific Reports

Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption. pubmed:27640363

Photodermatol Photoimmunol Photomed.

Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice pubmed:27995657

Molecular Medicine Reports

Icariin inhibits MMP‑1, MMP‑3 and MMP‑13 expression through MAPK pathways in IL‑1β‑stimulated SW1353 chondrosarcoma cells pubmed:28447732

bmc musculoskeletal disorders

Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. pubmed:28187731

Journal of Cellular Physiology

Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models pubmed:27739057

BMC Musculoskeletal Disorders

Establishment of rat ankle post-traumatic osteoarthritis model induced by malleolus fracture pubmed:29149841

Journal of Applied Animal Research

Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts:an in vitro approach to bovine digital dermatitis 10.108:09712119.2017.1335208

Alexandria Journal of Medicine

L-Carnitine ameliorates knee lesions in mono-iodoacetate induced osteoarthritis in rats 10.1016:j.ajme.2016.03.002

National Journal of Integrated Research in Medicine

Matrix Metalloproteinase-13 Can Be Used As an Independent Diagnostic and Prognostic Marker for Breast Carcinoma. AN=123116960

Molecular Medicine Reports

Effects of GGCX overexpression on anterior cruciate ligament transection-induced osteoarthritis in rabbits Pubmed:29257344

Journal of Applied Animal Research 

Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an approach to bovine digital dermatitis 10.1080:09712119.2017.1335208

Osteoarthritis Cartilage

Coordinated existence of multiple gangliosides is required for cartilage metabolism Pubmed: 30471358

PLoS One

Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis Pubmed: 30939166

Pathobiology

Suppressed Expression but Not Activity of Collagenases MMP-1 and MMP-13 in Human Renal Carcinoma Pubmed: 31129674

Bone

DAla2GIP antagonizes H2O2-induced chondrocyte apoptosis and inflammatory factor secretion Pubmed: 31283994

Food & Function

A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese …

JOURNAL OF CELLULAR BIOCHEMISTRY

Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes Pubmed: 32162384

Bone Research

Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis Pubmed: 32047705

Scientific Reports

Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis Pubmed: 31980722

Nutrients

Chondroprotective Properties of Human-Enriched Serum Following Polyphenol Extract Absorption: Results from an Exploratory Clinical Trial Pubmed: 31888255

Nature Communications

Vulnerability of progeroid smooth muscle cells to biomechanical forces is mediated by MMP13 Pubmed: 32807790

Molecular Medicine Reports

MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression Pubmed: 32705199

RESEARCH SQUARE

Experimental Observation of the Sequence of Tibial Plateau Chondrocyte and Matrix Degeneration in Spontaneous Osteoarthritis in Guinea Pigs


Hyaluronic Acid versus Alpha-2-Macroglobulin Intra-Articular Injections for Amelioration of Knee Posttraumatic Osteoarthritis: A Rat Model


Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs

Food Funct

Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice 33890588

Nutrients

Metabolic and Anti-Inflammatory Protective Properties of Human Enriched Serum Following Artichoke Leaf Extract Absorption: Results from an Innovative Ex Vivo … 34444810

Sci Rep

Liraglutide, a glucagon-like peptide 1 receptor agonist, exerts analgesic, anti-inflammatory and anti-degradative actions in osteoarthritis Pubmed:35091584


Catalog No.

Related products for research use of Mus musculus (Mouse) Organism species

Applications (RESEARCH USE ONLY!)

RPA099Mu01

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Positive Control; Immunogen; SDS-PAGE; WB.

RPA099Mu02

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Positive Control; Immunogen; SDS-PAGE; WB.

PAA099Mu01

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ELISA Kit for Matrix Metalloproteinase 13 (MMP13)

Enzyme-linked immunosorbent assay for Antigen Detection.

HEA099Mu

High Sensitive ELISA Kit for Matrix Metalloproteinase 13 (MMP13)

Enzyme-linked immunosorbent assay for Antigen Detection.

SCA099Mu

CLIA Kit for Matrix Metalloproteinase 13 (MMP13)

Chemiluminescent immunoassay for Antigen Detection.

LMA099Mu

Magnetic Luminex Assay Kit for Matrix Metalloproteinase 13 (MMP13) ,etc.

Magnetic Luminex Assay for Antigen Detection.

 

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