SKU(재고 관리 코드):PAA647Ra01
Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)
Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA647Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Homo sapiens (Human), Mus musculus (Mouse)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against Smad1. It has been selected for its ability to recognize Smad1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Magazine | Citations |
Chem Biol Interact | Camel milk attenuates the biochemical and morphological features of diabetic nephropathy: Inhibition of Smad1 and collagen type IV synthesis PubMed: 25617480 |
Research report | EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO files:p013-022 |
Phytomedicine | Efficacy of Total flavonoids of Rhizoma drynariae on the Blood Vessels and the Growth Quality of Bone Graft in the Induced Membrane Pubmed:35278899 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPA647Ra01 | Recombinant Mothers Against Decapentaplegic Homolog 1 (Smad1) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA647Ra01 | Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1) | WB; IHC; ICC; IP. |
LAA647Ra71 | Biotin-Linked Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1) | WB; IHC; ICC. |
LAA647Ra81 | FITC-Linked Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1) | WB; IHC; ICC; IF. |
MAA647Ra21 | Monoclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1) | WB; IHC; ICC; IP. |