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Cloud-clone

SKU(재고 관리 코드):PAA684Mu01

Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)

Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)

UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

PAA684Mu01

Organism Species

Mus musculus (Mouse) 

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA684Mu01-Recombinant Mucin 5 Subtype B (MUC5B)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

0.23mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MUC5B. It has been selected for its ability to recognize MUC5B in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
The Journal of Biological Chemistry The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via D-Prostanoid Receptor Signaling Jcb: 34199
The Journal of Biological Chemistry The ERK MAPK/RSK1 cascade phosphorylates CREB to induce MUC5B gene expression via DP1 receptor signaling Jcb: 247684
Respiratory Researh Pyocyanin-induced mucin production is associated with redox modification of FOXA2 PubMed: PMC3765780
Respirology Secreted mucins and airway bacterial colonization in non-CF bronchiectasis PubMed: 26172851
American Thoracic Society International Conference Abstracts Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness doi:10.1164
Ann Am Thorac Soc Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization doi:10.1513
Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой 10.20538/1682-0363-2017-2-146-158
Allergy Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps Pubmed:29802623
European Journal of Pharmacology  Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling Pubmed:29382534
Japan Society of Immunology & Allergology in Otolaryngology インフルエンザ菌Phosphorylcholine の表出と中耳ムチンMUC5AC 及びMUC5B 産生への影響 10.5648:jjiao.36.7
Journal of Investigative Medicine The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease Pubmed: 30573494
Mucosal Immunology ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism Pubmed: 31278375
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA684Mu02 Recombinant Mucin 5 Subtype B (MUC5B) Positive Control; Immunogen; SDS-PAGE; WB.
RPA684Mu01 Recombinant Mucin 5 Subtype B (MUC5B) Positive Control; Immunogen; SDS-PAGE; WB.
PAA684Mu01 Polyclonal Antibody to Mucin 5 Subtype B (MUC5B) WB; IHC; ICC; IP.
PAA684Mu02 Polyclonal Antibody to Mucin 5 Subtype B (MUC5B) WB; IHC; ICC; IP.
LAA684Mu81 FITC-Linked Polyclonal Antibody to Mucin 5 Subtype B (MUC5B) Applications: WB; IHC; ICC; IP.
LAA684Mu71 Biotin-Linked Polyclonal Antibody to Mucin 5 Subtype B (MUC5B) WB; IHC; ICC.
SEA684Mu ELISA Kit for Mucin 5 Subtype B (MUC5B) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA684Mu Magnetic Luminex Assay Kit for Mucin 5 Subtype B (MUC5B) ,etc. Magnetic Luminex Assay for Antigen Detection.
KSA684Mu01 ELISA Kit DIY Materials for Mucin 5 Subtype B (MUC5B) Main materials for "Do It (ELISA Kit) Yourself".

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