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SKU(재고 관리 코드):PAA181Ca01

Polyclonal Antibody to Neutrophil Elastase (NE)

Polyclonal Antibody to Neutrophil Elastase (NE)

UOM
Concentration
Organism species
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAA181Ca01

Organism Species

Canis familiaris; Canine (Dog).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA181Ca01-Recombinant Neutrophil Elastase (NE)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

Homo sapiens (Human)

Applications

WB; IHC

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NE. It has been selected for its ability to recognize NE in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Mediators of Inflammation Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation Hindawi: 612848
Journal of Photochemistry and Photobiology B: Biology A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins Pubmed:25732262
International Journal of Molecular Medicine The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats pubmed:27430552
European Journal of Trauma and Emergency Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. pubmed:28070608
Clin Exp Pharmacol Physiol. Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688
Journal of Cardiology Targeted regulation of sympathetic activity in paraventricular nucleus reduces inducible ventricular arrhythmias in rats after myocardial infarction 10.1016:j.jjcc.2018.06.003
Neuroscience Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats Pubmed:29684507
Molecular and Cellular Biochemistry Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes Pubmed:28948423
Neuropsychiatric disease and treatment The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic … Pubmed:29379286
Behavioural Brain Research Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats Pubmed: 30423388
Journal of Pineal Research Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice Pubmed: 30929267
Fundamental & clinical pharmacology Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivo Pubmed: 31313350
Cell Death & Disease Erythromycin suppresses neutrophil extracellular traps in smoking-related chronic pulmonary inflammation Pubmed: 31515489
Journal of nutritional science and vitaminology Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively … Pubmed: 31474682
Journal of Clinical Immunology MiR-608 Exerts Anti-inflammatory Effects by Targeting ELANE in Monocytes Pubmed: 31749032
Pathology & Oncology Research Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer Pubmed: 31656990
Bioscience Reports Inhibition of microRNA-146a attenuated heart failure in myocardial infarction rats Pubmed: 31763669
Oxidative Medicine and Cellular Longevity Neutrophil Elastase Inhibitors Suppress Oxidative Stress in Lung during Liver Transplantation Pubmed: 31871555
Cytokine Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460
Journal of neural transmission (Vienna, Austria : 1996) Neurotransmitter system aberrations in patients with drug addiction Pubmed: 32804296
experimental eye research Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis Pubmed: 32376471
CLINICAL AND EXPERIMENTAL HYPERTENSION Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats Pubmed: 32349626
Br J Pharmacol Macrophage Extracellular Traps Aggravate Iron Overload©\Related Liver Ischemia/Reperfusion Injury 33959955
Evidence-Based Complementary and Alternative Medicine Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling?¡­ 33505502
Nat Sci Sleep Depression of Non-Neuronal Cholinergic System May Play a Role in Co-Occurrence of Subjective Daytime Sleepiness and Hypertension in Patients with … 34934375
Neuromodulation: Technology at the Neural Interface Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome Pubmed:35088760
A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA181Ca01 Recombinant Neutrophil Elastase (NE) Positive Control; Immunogen; SDS-PAGE; WB.
PAA181Ca01 Polyclonal Antibody to Neutrophil Elastase (NE) WB; IHC
MAA181Ca21 Monoclonal Antibody to Neutrophil Elastase (NE) WB; IHC; ICC; IP.
SEA181Ca ELISA Kit for Neutrophil Elastase (NE) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA181Ca Magnetic Luminex Assay Kit for Neutrophil Elastase (NE) ,etc. Magnetic Luminex Assay for Antigen Detection.


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