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Cloud-clone

SKU(재고 관리 코드):PAB317Hu01

Polyclonal Antibody to Perforin 1 (PRF1)

Polyclonal Antibody to Perforin 1 (PRF1)

UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

PAB317Hu01

Organism Species

Homo sapiens (Human) 

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB317Hu01-Recombinant Perforin 1 (PRF1)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PRF1. It has been selected for its ability to recognize PRF1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Diagnostic Microbiology and Infectious Disease Granzyme B as a diagnostic marker of tuberculosis in patients with and without HIV coinfection Pubmed:26915636
FASEB JOURNAL The immunoregulatory effects of CD8 T-cell–derived perforin on diet-induced nonalcoholic steatohepatitis Pubmed: 30951375
Theranostics A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors Pubmed: 31695790
Mol Immunol CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection Pubmed: 33223223
INVESTIGATIONAL NEW DRUGS Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors Pubmed: 32772342
Am J Transl Res Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma 34377237
Nat Biomed Eng Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy 34873307
J Exp Med Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion Pubmed:35061012
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB317Hu02 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
APB317Hu01 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
RPB317Hu01 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
APB317Hu02 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
PAB317Hu01 Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
LAB317Hu81 FITC-Linked Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IF.
LAB317Hu71 Biotin-Linked Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC.
MAB317Hu22 Monoclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
MAB317Hu21 Monoclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
SEB317Hu ELISA Kit for Perforin 1 (PRF1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB317Hu Magnetic Luminex Assay Kit for Perforin 1 (PRF1) ,etc. Magnetic Luminex Assay for Antigen Detection.

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