Cloud-clone
SKU(재고 관리 코드):PAA588Ra01
Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA588Ra01
Organism Species
Rattus norvegicus (Rat)
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA588Ra01-Recombinant Pyruvate kinase isozymes M2 (PKM2)
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Homo sapiens (Human)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against PKM2. It has been selected for its ability to recognize PKM2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Cancer Research | Antigen Presentation by Dendritic Cells in Tumors Is Disrupted by Altered Metabolism that Involves Pyruvate Kinase M2 and Its Interaction with SOCS3 AACR: 70189 |
| Molecular BioSystems | Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer Pubmed:25483567 |
| Tumour Biol | M2 isoform of pyruvate kinase (PKM2) is upregulated in Kazakh’s ESCC and promotes proliferation and migration of ESCC cells PubMed: 26404132 |
| Oncotarget | Identification of a serum biomarker panel for the differential diagnosis of cholangiocarcinoma and primary sclerosing cholangitis Pubmed:29707118 |
| Journal of Neurotrauma | Acteoside improves muscle atrophy and motor function by inducing new myokine secretion in chronic spinal cord injury Pubmed: 30318996 |
| Cell Reports | Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling Pathway Pubmed: 32049010 |
| Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC) | |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212 |
| Life (Basel) | Putative Association between Low Baseline Gene Expression in the Peripheral Blood and Clinical Remission in Rheumatoid Arthritis Patients Treated with Tofacitinib 34947916 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA588Ra01 | Recombinant Pyruvate kinase isozymes M2 (PKM2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA588Ra01 | Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) | WB; IHC; ICC; IP. |
| FAA588Ra01 | Anti-Pyruvate kinase isozymes M2 (PKM2) Polyclonal Antibody | Flow cytometry. |
| MAA588Ra21 | Monoclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) | WB; IHC; ICC; IP. |
| SEA588Ra | ELISA Kit for Pyruvate kinase isozymes M2 (PKM2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA588Ra | Magnetic Luminex Assay Kit for Pyruvate kinase isozymes M2 (PKM2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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