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Cloud-clone

SKU(재고 관리 코드):PAL920Hu01

Polyclonal Antibody to Semaphorin 3E (SEMA3E)

Polyclonal Antibody to Semaphorin 3E (SEMA3E)

UOM
Concentration
Organism species
Western blotting: 0.5-2ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.

Product No.

PAL920Hu01

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPL920Hu01-Recombinant Semaphorin 3E (SEMA3E)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SEMA3E. It has been selected for its ability to recognize SEMA3E in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-3µg/mL
Immunohistochemistry: 5-30µg/mL
Immunocytochemistry: 5-30µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Ophthalmology Aqueous Levels of Angiopoietin-like 4 and Semaphorin 3E Correlate with Nonperfusion Area and Macular Volume in Diabetic Retinopathy PubMed: 25687026
annals of clinical and laboratory science Association of Increased Serum Sema3E with TRIB3 Q84R Polymorphism and Carotid Atherosclerosis in Metabolic Syndrome. pubmed:28249916
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE Spatial association of SEMA3C with nerve endings/terminal Schwann cells in hair follicle isthmus region Pubmed: 32954569
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patients Pubmed: 32569480
EMBO JOURNAL RhoJ integrates attractive and repulsive cues in directional migration of endothelial cells Pubmed: 32347571
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPL920Hu01 Recombinant Semaphorin 3E (SEMA3E) Positive Control; Immunogen; SDS-PAGE; WB.
PAL920Hu01 Polyclonal Antibody to Semaphorin 3E (SEMA3E) WB; IHC; ICC; IP.
MAL920Hu21 Monoclonal Antibody to Semaphorin 3E (SEMA3E) WB; IHC; ICC; IP.
SEL920Hu ELISA Kit for Semaphorin 3E (SEMA3E) Enzyme-linked immunosorbent assay for Antigen Detection.
LML920Hu Magnetic Luminex Assay Kit for Semaphorin 3E (SEMA3E) ,etc. Magnetic Luminex Assay for Antigen Detection.

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