Cloud-clone
SKU(재고 관리 코드):PAF396Ra01
Polyclonal Antibody to Spondin 2 (SPON2)
Polyclonal Antibody to Spondin 2 (SPON2)
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAF396Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against SPON2. It has been selected for its ability to recognize SPON2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| The Journal of Urology | Spondin-2, a secreted extracellular matrix protein, is a novel diagnostic biomarker for prostate cancer Pubmed: 23665271 |
| Oxford Journals | SP136EVALUATION OF SERUM SPONDIN 2 LEVELS IN THE DIFFERENT ETIOLOGIES OF GLOMERULAR DISEASES Content: 30 |
| Nephrology (Carlton) | Evaluation of Serum Spondin 2 Levels in the Different Stages of Type 2 Diabetic Nephropathy PubMed: 25973958 |
| Oncogene | The extracellular matrix protein mindin attenuates colon cancer progression by blocking angiogenesis via Egr-1-mediated regulation pubmed:28991232 |
| Endocrinology | Study of human T21 placenta suggests a potential role of mesenchymal spondin-2 in placental vascular development Pubmed: 30715257 |
| Journal of Cellular and Molecular Medicine | Mindin serves as a tumour suppressor gene during colon cancer progression through MAPK/ERK signalling pathway in mice Pubmed: 32614521 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPF396Ra01 | Recombinant Spondin 2 (SPON2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF396Ra01 | Polyclonal Antibody to Spondin 2 (SPON2) | WB; IHC; ICC; IP. |
| MAF396Ra21 | Monoclonal Antibody to Spondin 2 (SPON2) | WB; IHC; ICC; IP. |
| SEF396Ra | ELISA Kit for Spondin 2 (SPON2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF396Ra | Magnetic Luminex Assay Kit for Spondin 2 (SPON2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
Share
