Cloud-clone
SKU(재고 관리 코드):PAA133Mu01
Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.
Product No.
PAA133Mu01
Organism Species
Mus musculus (Mouse).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA133Mu01-Recombinant Tumor Necrosis Factor Alpha (TNFa)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.5mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-40µg/mL;
Immunocytochemistry: 5-40µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Not available | Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia Universi: 49434630 |
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| The European Journal of Clinical Investigation | Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes Wiley: source |
| Journal of Biomedical Research | Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy ScienceDirect: S1674830111600158 |
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| Journal of Vascular and Interventional Radiology | Hepatocellular Necrosis, Apoptosis, and Proliferation after Transcatheter Arterial Embolization or Chemoembolization in a Standardized Rabbit Model PubMed: 21959058 |
| Bone | Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration PubMed: 22796416 |
| Journal of Periodontology | The Effects of a Novel Botanical Agent on LPS-Induced Alveolar Bone Loss in Rats PubMed: 23106504 |
| Ann Rheum Dis. | The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats PubMed: 23172753 |
| Journal of Surgical Research | Enhanced tumor growth after portal vein embolization in a rabbit tumor model PubMed: 23149224 |
| Journal of Applied Physiology | Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process PubMed: 22984252 |
| Journal of Periodontology Online | The Effect of Radachlorin-Mediated Antimicrobial Photodynamic Therapy on Clinical Parameters and Cytokine Profile in Ligature-Induced Periodontitis in Dogs PubMed: 22813345 |