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Cloud-clone
SKU(재고 관리 코드):PAA133Ov01
Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
PAA133Ov01
Organism Species
Ovis aries; Ovine (Sheep).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA133Ov01-Recombinant Tumor Necrosis Factor Alpha (TNFa)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Experimental Animals | Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial Infarction Jstage: source |
| Not available | Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia Universi: 49434630 |
| 41 | Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. PubMed: 20522592 |
| Respiration | Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice Karger: 000319151 |
| Journal of Huazhong University of Science and Technology-Medical Sciences | Expression and significance of fgl2 prothrombinase in cardiac microvascular endothelial cells of rats with type 2 diabetes SpringerLink: 161m725t24x85kvv |
| The European Journal of Clinical Investigation | Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes Wiley: source |
| Journal of Biomedical Research | Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy ScienceDirect: S1674830111600158 |
| Stem Cells | Combined Effects of Hematopoietic Progenitor Cell Mobilization from Bone Marrow by Granulocyte Colony Stimulating Factor and AMD3100 and Chemotaxis into the Brain Using Stromal Cell-Derived Factor-1α in an Alzheimer's Disease Mouse Model PubMed: 21608078 |
| Journal of Physiology and Biochemistry | Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats SpringerLink: h875w73171w2147w |
| The EMBO Journal | Egr-1 decreases adipocyte insulin sensitivity by tilting PI3K/Akt and MAPK signal balance in mice Nature: 2011277 |
| Reproductive BioMedicine Online | Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages ScienceDirect: S1472648312001484 |
| Journal of Vascular and Interventional Radiology | Hepatocellular Necrosis, Apoptosis, and Proliferation after Transcatheter Arterial Embolization or Chemoembolization in a Standardized Rabbit Model PubMed: 21959058 |
| Bone | Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration PubMed: 22796416 |
| Journal of Periodontology | The Effects of a Novel Botanical Agent on LPS-Induced Alveolar Bone Loss in Rats PubMed: 23106504 |
| Ann Rheum Dis. | The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats PubMed: 23172753 |
| Journal of Surgical Research | Enhanced tumor growth after portal vein embolization in a rabbit tumor model PubMed: 23149224 |
| Journal of Applied Physiology | Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process PubMed: 22984252 |
| Journal of Periodontology Online | The Effect of Radachlorin-Mediated Antimicrobial Photodynamic Therapy on Clinical Parameters and Cytokine Profile in Ligature-Induced Periodontitis in Dogs PubMed: 22813345 |
| PLoS One | New Method to Isolate and Culture Rat Kupffer Cells PubMed: PMC3743898 |
| PLoS One | Recombinant Adiponectin Ameliorates Liver Ischemia Reperfusion Injury via Activating the AMPK/eNOS Pathway PubMed: PMC3676360 |
| The Open Respiratory Medicine Journal | Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias PubMed: PMC3551240 |
| Experimental and Therapeutic Medicine | Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κB PubMed: PMC3570159 |
| Journal of Ocular Pharmacology and Therapeutics | Comparison of Acute Effect of Systemic Versus Intravitreal Infliximab Treatment in an Experimental Model of Endotoxin-Induced Uveitis Pubmed: 24116865 |
| Polish Journal of Veterinary Sciences | The effect of different dietary levels of vitamin E and selenium on antioxidant status and immunological markers in serum of laying hens Pubmed: 23971202 |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
| International Immunopharmacology | Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis Pubmed: 24126114 |
| Neurochemical Research | Neonatal morphine administration leads to changes in hippocampal BDNF levels and antioxidant enzyme activity in the adult life of rats Pubmed: 23224818 |
| Journal of natural remedies | Ixora coccinea down Regulate the Inflammation-Associated Prostaglandins (PGE-2), Nitric Oxide (NO) and Cytokines Production in LPS-Stimulated RAW 264.7 Macrophage Jnronline: Source |
| Inflamm Res. | Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats. Pubmed: 24352531 |
| PLoS One. | Megakaryocytic leukemia 1 (MKL1) regulates hypoxia induced pulmonary hypertension in rats. Pubmed: 24647044 |
| J Surg Res. | Immunogenic alteration in laparoscopic common bile duct exploration. Pubmed: 24239148 |
| BMC anesthesiology | Repeated open endotracheal suctioning causes gradual desaturation but does not exacerbate lung injury compared to closed endotracheal suctioning in a rabbit model of ARDS. Pubmed: 24308643 |
| Physiological Research | Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome. Pubmed: 2432969 |
| Mutation Research/Genetic Toxicology and Environmental Mutagenesis | Genotoxicity of intraperitoneal injection of lipoamphiphile CdSe/ZnS quantum dots in rats Pubmed: 24055877 |
| Immunology letters | Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection. Pubmed: 24286726 |
| Journal of Stroke and Cerebrovascular Diseases | Activation of Peroxisome Proliferator-activated Receptor β/δ Attenuates Acute Ischemic Stroke on Middle Cerebral Ischemia Occlusion in Rats. Pubmed: 24774438 |
| Journal of Materials Chemistry B | Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering Rsc: Source |
| International Immunopharmacology | Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits ScienceDirect: S1567576913004153 |
| Journal of Pharmaceutical Sciences and Pharmacology | Anti-inflammatory Effect of Euphorbia hirta Leaf Extract in Rats and Modulation of Inflammation-Associated Prostaglandins (PGE-2) and Nitric Oxide (NO) Expression in RAW264.7 Macrophage Ingentaconnect: Source |
| Veterinary Research | Immunosuppression abrogates resistance of young rabbits to Rabbit Haemorrhagic Disease (RHD) Biomedcentral: Source |
| Journal of Periodontology | Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial Joponline: 130464 |
| Molecular and Cellular Endocrinology | Nonalcoholic steatohepatitis as a novel player in metabolic syndrome-induced erectile dysfunction: An experimental study in the rabbit Pubmed:24486698 |
| Toxicol Appl Pharmacol.? | Amelioration of nandrolone decanoate-induced testicular and sperm toxicity in rats by taurine: Effects on steroidogenesis, redox and inflammatory cascades, and intrinsic apoptotic pathway Pubmed:25542992 |
| Journal of Traditional and Complementary Medicine | Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats Sciencedirect:S2225411015000231 |
| INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH | L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION Tdmu:Source |
| New Journal of Chemistry | Biophysico-chemical interfacial attributes of Fe3O4 decorated MWCNT nanohybrid/bio-based hyperbranched polyurethane nanocomposite: an antibacterial wound healing material with controlled drug release potential Rsc:Source |
| Evidence-Based Complementary and Alternative Medicine | Ginsenosides Rg1 from Panax ginseng: A Potential Therapy for Acute Liver Failure Patients? Pubmed:Pmc4241314 |
| Exp Ther Med. | PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosis Pubmed:Pmc4079423 |
| PLoS One. | Ginsenoside Rg1 prevents cognitive impairment and hippocampus senescence in a rat model of d-galactose-induced aging Pubmed:Pmc4076296 |
| Int J Chron Obstruct Pulmon Dis. | Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease Pubmed:Pmc4043430 |
| PLOS ONE | Megakaryocytic Leukemia 1 (MKL1) Regulates Hypoxia Induced Pulmonary Hypertension in Rats Pubmed:Pmc3960100 |
| J Gastroenterol Hepatol.? | Hypothermia induced by adenosine 5′‐monophosphate attenuates injury in an L‐arginine‐induced acute pancreatitis rat model Pubmed:24224980 |
| Immunol Lett. | Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection Pubmed:24286726 |
| Inflamm Res. | Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats Pubmed:24352531 |
| Inflammation | Effects of honokiol on sepsis-induced acute kidney injury in an experimental model of sepsis in rats Pubmed:24531855 |
| PLOS ONE | Metformin Inhibits the IL-6-Induced Epithelial-Mesenchymal Transition and Lung Adenocarcinoma Growth and Metastasis Pubmed:24789104 |
| British Journal of Nutrition | Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steers Pubmed:24846452 |
| Journal of Environmental Pathology, Toxicology and Oncology | Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways Pubmed:24941292 |
| J Surg Res. | Valproic acid protects septic mice from renal injury by reducing the inflammatory response Pubmed:24948542 |
| Inflammation.? | Alpha-lipoic acid exerts anti-inflammatory effects on lipopolysaccharide-stimulated rat mesangial cells via inhibition of nuclear factor kappa B (NF-κB) signaling pathway. Pubmed:24962643 |
| Archives of Oral Biology | Hyperlipidemia causes changes in inflammatory responses to periodontal pathogen challenge: Implications in acute and chronic infections Pubmed:24992577 |
| Free Radical Biology and Medicine | Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835 |
| European Review for Medical and Pharmacological Sciences | The anti-inflammatory effects of 1,1 dimethyl-4- phenylpiperazinium (DMPP) compared to dexamethasone in a guinea pig model of ovalbumin induced asthma Pubmed:25070830 |
| European Journal of Pharmacology | Neuroprotective effects of rosuvastatin against traumatic spinal cord injury in rats Pubmed:25084223 |
| Inflammation. | Oral administration?of?Saccharomyces?boulardii ameliorates carbon tetrachloride-induced liver fibrosis in?rats?via reducing intestinal permeability and modulating gut microbial composition. Pubmed:25227279 |
| PLOS ONE | Differential Pro-Inflammatory Responses of Astrocytes and Microglia Involve STAT3 Activation in Response to 1800 MHz Radiofrequency Fields Pubmed:25275372 |
| Journal of Obstetrics and Gynaecology Research | Regression of experimental endometriotic implants in a rat model with the angiotensin II receptor blocker losartan Pubmed:25302540 |
| Food & Function | Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice Pubmed:25308407 |
| PLOS ONE | Bisphenol A Exposure Enhances Atherosclerosis in WHHL Rabbits Pubmed:25333893 |
| Oncotarget | Senescence of tumor cells induced by oxaliplatin increases the efficiency of a lipid A immunotherapy via the recruitment of neutrophils Pubmed:25347345 |
| Molecular Medicine Reports | GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes. Pubmed:25377925 |
| Neural Regen Res.? | Inflammatory response and neuronal necrosis in rats with cerebral ischemia Pubmed:25422636 |
| Life Sciences | Adipose tissue regulates hepatic cholesterol metabolism via adiponectin Pubmed:25445438 |
| International Immunopharmacology | NF-κB activation and proinflammatory cytokines mediated protective effect of Indigofera caerulea Roxb. on CCl 4 induced liver damage in rats Pubmed:25445959 |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261 |
| Journal of Surgical Research | Ursolic acid improves survival and attenuates lung injury in septic rats induced by cecal ligation and puncture Pubmed:25454976 |
| Journal of Surgical Research | Melatonin attenuates intestinal ischemia–reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2 Pubmed:25491174 |
| Indian J Pharmacol | Effect of coenzyme Q10 alone and its combination with metformin on streptozotocin-nicotinamide-induced diabetic nephropathy in rats Pubmed:25538335 |
| Chemical Research in Toxicology | Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880 |
| Neurochemistry International | Sulfiredoxin-1 protects primary cultured astrocytes from ischemia-induced damage Pubmed:25620665 |
| Journal of Translational Medicine | Protective effects of astaxanthin against ischemia/reperfusion induced renal injury in mice Pubmed:25623758 |
| Molecular Medicine Reports | Tanshinone ⅡA attenuates bleomycin-induced pulmonary fibrosis in rats Pubmed:25672255 |
| Reproduction in Domestic Animals | The Concentrations of Inflammatory Cytokines and Acute‐Phase Proteins in the Peripheral Blood and Uterine Washings in Cows with Pyometra Pubmed:25704413 |
| Journal of Surgical Research | Exogenous carcinoembryonic antigen-related cell adhesion molecule 1 suppresses 2,4,6-trinitrobenzene sulfonic acid-induced ulcerative colitis in mice Pubmed:25724769 |
| Internat |