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Cloud-clone

SKU(재고 관리 코드):PAA133Ov01

Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Product No.

PAA133Ov01

Organism Species

Ovis aries; Ovine (Sheep).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA133Ov01-Recombinant Tumor Necrosis Factor Alpha (TNFa)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Experimental Animals Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial Infarction Jstage: source
Not available Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia Universi: 49434630
41 Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. PubMed: 20522592
Respiration Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice Karger: 000319151
Journal of Huazhong University of Science and Technology-Medical Sciences Expression and significance of fgl2 prothrombinase in cardiac microvascular endothelial cells of rats with type 2 diabetes SpringerLink: 161m725t24x85kvv
The European Journal of Clinical Investigation Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes Wiley: source
Journal of Biomedical Research Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy ScienceDirect: S1674830111600158
Stem Cells Combined Effects of Hematopoietic Progenitor Cell Mobilization from Bone Marrow by Granulocyte Colony Stimulating Factor and AMD3100 and Chemotaxis into the Brain Using Stromal Cell-Derived Factor-1α in an Alzheimer's Disease Mouse Model PubMed: 21608078
Journal of Physiology and Biochemistry Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats SpringerLink: h875w73171w2147w
The EMBO Journal Egr-1 decreases adipocyte insulin sensitivity by tilting PI3K/Akt and MAPK signal balance in mice Nature: 2011277
Reproductive BioMedicine Online Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages ScienceDirect: S1472648312001484
Journal of Vascular and Interventional Radiology Hepatocellular Necrosis, Apoptosis, and Proliferation after Transcatheter Arterial Embolization or Chemoembolization in a Standardized Rabbit Model PubMed: 21959058
Bone Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration PubMed: 22796416
Journal of Periodontology The Effects of a Novel Botanical Agent on LPS-Induced Alveolar Bone Loss in Rats PubMed: 23106504
Ann Rheum Dis. The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats PubMed: 23172753
Journal of Surgical Research Enhanced tumor growth after portal vein embolization in a rabbit tumor model PubMed: 23149224
Journal of Applied Physiology Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process PubMed: 22984252
Journal of Periodontology Online The Effect of Radachlorin-Mediated Antimicrobial Photodynamic Therapy on Clinical Parameters and Cytokine Profile in Ligature-Induced Periodontitis in Dogs PubMed: 22813345
PLoS One New Method to Isolate and Culture Rat Kupffer Cells PubMed: PMC3743898
PLoS One Recombinant Adiponectin Ameliorates Liver Ischemia Reperfusion Injury via Activating the AMPK/eNOS Pathway PubMed: PMC3676360
The Open Respiratory Medicine Journal Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias PubMed: PMC3551240
Experimental and Therapeutic Medicine Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κB PubMed: PMC3570159
Journal of Ocular Pharmacology and Therapeutics Comparison of Acute Effect of Systemic Versus Intravitreal Infliximab Treatment in an Experimental Model of Endotoxin-Induced Uveitis Pubmed: 24116865
Polish Journal of Veterinary Sciences The effect of different dietary levels of vitamin E and selenium on antioxidant status and immunological markers in serum of laying hens Pubmed: 23971202
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
International Immunopharmacology Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis Pubmed: 24126114
Neurochemical Research Neonatal morphine administration leads to changes in hippocampal BDNF levels and antioxidant enzyme activity in the adult life of rats Pubmed: 23224818
Journal of natural remedies Ixora coccinea down Regulate the Inflammation-Associated Prostaglandins (PGE-2), Nitric Oxide (NO) and Cytokines Production in LPS-Stimulated RAW 264.7 Macrophage Jnronline: Source
Inflamm Res. Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats. Pubmed: 24352531
PLoS One. Megakaryocytic leukemia 1 (MKL1) regulates hypoxia induced pulmonary hypertension in rats. Pubmed: 24647044
J Surg Res. Immunogenic alteration in laparoscopic common bile duct exploration. Pubmed: 24239148
BMC anesthesiology Repeated open endotracheal suctioning causes gradual desaturation but does not exacerbate lung injury compared to closed endotracheal suctioning in a rabbit model of ARDS. Pubmed: 24308643
Physiological Research Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome. Pubmed: 2432969
Mutation Research/Genetic Toxicology and Environmental Mutagenesis Genotoxicity of intraperitoneal injection of lipoamphiphile CdSe/ZnS quantum dots in rats Pubmed: 24055877
Immunology letters Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection. Pubmed: 24286726
Journal of Stroke and Cerebrovascular Diseases Activation of Peroxisome Proliferator-activated Receptor β/δ Attenuates Acute Ischemic Stroke on Middle Cerebral Ischemia Occlusion in Rats. Pubmed: 24774438
Journal of Materials Chemistry B Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering Rsc: Source
International Immunopharmacology Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits ScienceDirect: S1567576913004153
Journal of Pharmaceutical Sciences and Pharmacology Anti-inflammatory Effect of Euphorbia hirta Leaf Extract in Rats and Modulation of Inflammation-Associated Prostaglandins (PGE-2) and Nitric Oxide (NO) Expression in RAW264.7 Macrophage Ingentaconnect: Source
Veterinary Research Immunosuppression abrogates resistance of young rabbits to Rabbit Haemorrhagic Disease (RHD) Biomedcentral: Source
Journal of Periodontology Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial Joponline: 130464
Molecular and Cellular Endocrinology Nonalcoholic steatohepatitis as a novel player in metabolic syndrome-induced erectile dysfunction: An experimental study in the rabbit Pubmed:24486698
Toxicol Appl Pharmacol.? Amelioration of nandrolone decanoate-induced testicular and sperm toxicity in rats by taurine: Effects on steroidogenesis, redox and inflammatory cascades, and intrinsic apoptotic pathway Pubmed:25542992
Journal of Traditional and Complementary Medicine Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats Sciencedirect:S2225411015000231
INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION Tdmu:Source
New Journal of Chemistry Biophysico-chemical interfacial attributes of Fe3O4 decorated MWCNT nanohybrid/bio-based hyperbranched polyurethane nanocomposite: an antibacterial wound healing material with controlled drug release potential Rsc:Source
Evidence-Based Complementary and Alternative Medicine Ginsenosides Rg1 from Panax ginseng: A Potential Therapy for Acute Liver Failure Patients? Pubmed:Pmc4241314
Exp Ther Med. PAR2, IL4R, TGFβ and TNFα in bronchoalveolar lavage distinguishes extrinsic allergic alveolitis from sarcoidosis Pubmed:Pmc4079423
PLoS One. Ginsenoside Rg1 prevents cognitive impairment and hippocampus senescence in a rat model of d-galactose-induced aging Pubmed:Pmc4076296
Int J Chron Obstruct Pulmon Dis. Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease Pubmed:Pmc4043430
PLOS ONE Megakaryocytic Leukemia 1 (MKL1) Regulates Hypoxia Induced Pulmonary Hypertension in Rats Pubmed:Pmc3960100
J Gastroenterol Hepatol.? Hypothermia induced by adenosine 5′‐monophosphate attenuates injury in an L‐arginine‐induced acute pancreatitis rat model Pubmed:24224980
Immunol Lett. Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection Pubmed:24286726
Inflamm Res. Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats Pubmed:24352531
Inflammation Effects of honokiol on sepsis-induced acute kidney injury in an experimental model of sepsis in rats Pubmed:24531855
PLOS ONE Metformin Inhibits the IL-6-Induced Epithelial-Mesenchymal Transition and Lung Adenocarcinoma Growth and Metastasis Pubmed:24789104
British Journal of Nutrition Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steers Pubmed:24846452
Journal of Environmental Pathology, Toxicology and Oncology Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways Pubmed:24941292
J Surg Res. Valproic acid protects septic mice from renal injury by reducing the inflammatory response Pubmed:24948542
Inflammation.? Alpha-lipoic acid exerts anti-inflammatory effects on lipopolysaccharide-stimulated rat mesangial cells via inhibition of nuclear factor kappa B (NF-κB) signaling pathway. Pubmed:24962643
Archives of Oral Biology Hyperlipidemia causes changes in inflammatory responses to periodontal pathogen challenge: Implications in acute and chronic infections Pubmed:24992577
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European Review for Medical and Pharmacological Sciences The anti-inflammatory effects of 1,1 dimethyl-4- phenylpiperazinium (DMPP) compared to dexamethasone in a guinea pig model of ovalbumin induced asthma Pubmed:25070830
European Journal of Pharmacology Neuroprotective effects of rosuvastatin against traumatic spinal cord injury in rats Pubmed:25084223
Inflammation. Oral administration?of?Saccharomyces?boulardii ameliorates carbon tetrachloride-induced liver fibrosis in?rats?via reducing intestinal permeability and modulating gut microbial composition. Pubmed:25227279
PLOS ONE Differential Pro-Inflammatory Responses of Astrocytes and Microglia Involve STAT3 Activation in Response to 1800 MHz Radiofrequency Fields Pubmed:25275372
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Food & Function Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice Pubmed:25308407
PLOS ONE Bisphenol A Exposure Enhances Atherosclerosis in WHHL Rabbits Pubmed:25333893
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Molecular Medicine Reports GYY4137, a hydrogen sulfide‑releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor‑kappa B and mitogen‑activated protein kinases in Coxsackie virus B3‑infected rat cardiomyocytes. Pubmed:25377925
Neural Regen Res.? Inflammatory response and neuronal necrosis in rats with cerebral ischemia Pubmed:25422636
Life Sciences Adipose tissue regulates hepatic cholesterol metabolism via adiponectin Pubmed:25445438
International Immunopharmacology NF-κB activation and proinflammatory cytokines mediated protective effect of Indigofera caerulea Roxb. on CCl 4 induced liver damage in rats Pubmed:25445959
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis Pubmed:25448261
Journal of Surgical Research Ursolic acid improves survival and attenuates lung injury in septic rats induced by cecal ligation and puncture Pubmed:25454976
Journal of Surgical Research Melatonin attenuates intestinal ischemia–reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2 Pubmed:25491174
Indian J Pharmacol Effect of coenzyme Q10 alone and its combination with metformin on streptozotocin-nicotinamide-induced diabetic nephropathy in rats Pubmed:25538335
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880
Neurochemistry International Sulfiredoxin-1 protects primary cultured astrocytes from ischemia-induced damage Pubmed:25620665
Journal of Translational Medicine Protective effects of astaxanthin against ischemia/reperfusion induced renal injury in mice Pubmed:25623758
Molecular Medicine Reports Tanshinone ⅡA attenuates bleomycin-induced pulmonary fibrosis in rats Pubmed:25672255
Reproduction in Domestic Animals The Concentrations of Inflammatory Cytokines and Acute‐Phase Proteins in the Peripheral Blood and Uterine Washings in Cows with Pyometra Pubmed:25704413
Journal of Surgical Research Exogenous carcinoembryonic antigen-related cell adhesion molecule 1 suppresses 2,4,6-trinitrobenzene sulfonic acid-induced ulcerative colitis in mice Pubmed:25724769
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