SKU(재고 관리 코드):PAF557Ra01
Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)
Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)
Western blotting: 0.5-2µg/mL;1:140-560
Immunohistochemistry: 5-20µg/mL;1:14-56
Immunocytochemistry: 5-20µg/mL;1:14-56
Optimal working dilutions must be determined by end user.
Product No.
PAF557Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPF557Ra01-Recombinant Uncoupling Protein 1, Mitochondrial (UCP1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.28mg/ml
Organism Species More
Mus musculus (Mouse)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against UCP1. It has been selected for its ability to recognize UCP1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Magazine |
Citations |
Stem Cells |
FM19G11 Favors Spinal Cord Injury Regeneration and Stem Cell Self-Renewal by Mitochondrial Uncoupling and Glucose Metabolism Induction PubMed: 22865656 |
Diabetes. |
Inorganic nitrate promotes the browning of white adipose tissue through the nitrate-nitrite-nitric oxide pathway Pubmed:25249574 |
Journal of Cachexia, Sarcopenia and Muscle |
Muscle wasting and adipose tissue browning in infantile nephropathic cystinosis Doi: 10.1002 |
FASEB J |
Single-cell transcriptomics and functional target validation of brown adipocytes show their complex roles in metabolic homeostasis PubMed: 26304220 |
FASEB JOURNAL |
Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and … Pubmed: 32954574 |
Brown and beige adipose tissue regulate systemic metabolism to resist diet-induced obesity through metabolite signals in an inter-organ signaling axis |
|
Science reports |
All-trans-retinoic acid ameliorates atherosclerosis, promotes perivascular adipose tissue browning, and increases adiponectin production in Apo-E mice 33627760 |
Nature Communications |
Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis 33772024 |
Catalog No. |
Related products for research use of Rattus norvegicus (Rat) Organism species |
Applications (RESEARCH USE ONLY!) |
RPF557Ra01 |
Positive Control; Immunogen; SDS-PAGE; WB. |
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RPF557Ra02 |
Positive Control; Immunogen; SDS-PAGE; WB. |
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PAF557Ra01 |
Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1) |
WB; IHC; ICC; IP. |
PAF557Ra02 |
Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1) |
WB; IHC; ICC; IP. |
LAF557Ra71 |
Biotin-Linked Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1) |
WB; IHC; ICC. |
SEF557Ra |
Enzyme-linked immunosorbent assay for Antigen Detection. |
|
LMF557Ra |
Magnetic Luminex Assay Kit for Uncoupling Protein 1, Mitochondrial (UCP1) ,etc. |
Magnetic Luminex Assay for Antigen Detection. |