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SKU(재고 관리 코드):PAF557Ra01

Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)

Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)

UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;1:140-560
Immunohistochemistry: 5-20µg/mL;1:14-56
Immunocytochemistry: 5-20µg/mL;1:14-56
Optimal working dilutions must be determined by end user.

Product No.

PAF557Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPF557Ra01-Recombinant Uncoupling Protein 1, Mitochondrial (UCP1)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

0.28mg/ml

Organism Species More

Mus musculus (Mouse)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against UCP1. It has been selected for its ability to recognize UCP1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Stem Cells

FM19G11 Favors Spinal Cord Injury Regeneration and Stem Cell Self-Renewal by Mitochondrial Uncoupling and Glucose Metabolism Induction PubMed: 22865656

Diabetes.

Inorganic nitrate promotes the browning of white adipose tissue through the nitrate-nitrite-nitric oxide pathway Pubmed:25249574

Journal of Cachexia, Sarcopenia and Muscle

Muscle wasting and adipose tissue browning in infantile nephropathic cystinosis Doi: 10.1002

FASEB J

Single-cell transcriptomics and functional target validation of brown adipocytes show their complex roles in metabolic homeostasis PubMed: 26304220

FASEB JOURNAL

Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and … Pubmed: 32954574


Brown and beige adipose tissue regulate systemic metabolism to resist diet-induced obesity through metabolite signals in an inter-organ signaling axis

Science reports

All-trans-retinoic acid ameliorates atherosclerosis, promotes perivascular adipose tissue browning, and increases adiponectin production in Apo-E mice 33627760

Nature Communications

Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis 33772024


Catalog No.

Related products for research use of Rattus norvegicus (Rat) Organism species

Applications (RESEARCH USE ONLY!)

RPF557Ra01

Recombinant Uncoupling Protein 1, Mitochondrial (UCP1)

Positive Control; Immunogen; SDS-PAGE; WB.

RPF557Ra02

Recombinant Uncoupling Protein 1, Mitochondrial (UCP1)

Positive Control; Immunogen; SDS-PAGE; WB.

PAF557Ra01

Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)

WB; IHC; ICC; IP.

PAF557Ra02

Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)

WB; IHC; ICC; IP.

LAF557Ra71

Biotin-Linked Polyclonal Antibody to Uncoupling Protein 1, Mitochondrial (UCP1)

WB; IHC; ICC.

SEF557Ra

ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1)

Enzyme-linked immunosorbent assay for Antigen Detection.

LMF557Ra

Magnetic Luminex Assay Kit for Uncoupling Protein 1, Mitochondrial (UCP1) ,etc.

Magnetic Luminex Assay for Antigen Detection.

 

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