PKNOX2; PREP2; Homeobox protein PKNOX2; Homeobox protein PREP-2; PBX/knotted homeobox 2
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human PKNOX2. AA range:341-390
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
55kD
GeneID（Human）:
63876
Human Swiss-Prot No:
Q96KN3
Cellular localization:
Nucleus .
Background:
Homeodomain proteins are sequence-specific transcription factors that share a highly conserved DNA-binding domain and play fundamental roles in cell proliferation, differentiation, and death. PKNOX2 belongs to the TALE (3-amino acid loop extension) class of homeodomain proteins characterized by a 3-amino acid extension between alpha helices 1 and 2 within the homeodomain (Imoto et al., 2001 [PubMed 11549286]).[supplied by OMIM, Oct 2009],
Western Blot analysis of various cells using PREP-2 Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor
Western blot analysis of lysates from HepG2 cells, using PKNOX2 Antibody. The lane on the right is blocked with the synthesized peptide.