Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human RAB2A. AA range:84-133
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
25kD
GeneID（Human）:
5862
Human Swiss-Prot No:
P61019
Cellular localization:
Endoplasmic reticulum-Golgi intermediate compartment membrane ; Lipid-anchor . Melanosome . Endoplasmic reticulum membrane ; Lipid-anchor . Golgi apparatus membrane ; Lipid-anchor . Cytoplasmic vesicle, secretory vesicle, acrosome . Localized in the Golgi apparatus in the round spermatids and in the acrosome in the elongating spermatid (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). .
Background:
The protein encoded by this gene belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
Western Blot analysis of A549 cells using Rab 2A Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).