1 1

ELK Biotechnology

ELK9678

Rabbit LAMP2(Lysosomal Associated Membrane Protein 2) ELISA Kit

Rabbit LAMP2(Lysosomal Associated Membrane Protein 2) ELISA Kit

Sensitivity

Rabbit LAMP2(Lysosomal Associated Membrane Protein 2) ELISA Kit

Overview

Product name: Rabbit LAMP2(Lysosomal Associated Membrane Protein 2) ELISA Kit
Reactivity: Rabbit
Alternative Names: CD107-B; CD107b; LAMP-2C; LAMPB; LGP96; Lysosome-associated membrane glycoprotein 2; CD107 antigen-like family member B
Assay Type: Sandwich
Sensitivity: 24.9 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit LAMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit LAMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit LAMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit LAMP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
4000.00 1.983 1.896
2000.00 1.576 1.489
1000.00 1.128 1.041
500.00 0.848 0.761
250.00 0.518 0.431
125.00 0.312 0.225
62.50 0.226 0.139
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant LAMP2 and the recovery rates were calculated by comparing the measured value to the expected amount of LAMP2 in samples.
Matrix Recovery range Average
serum(n=5) 84-98% 91%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 94-107% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LAMP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-99% 80-96% 91-101% 89-100%
EDTA plasma(n=5) 79-91% 89-103% 83-96% 86-95%
Heparin plasma(n=5) 90-102% 87-98% 87-95% 85-96%
전체 세부 정보 보기