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ELK Biotechnology

ELK9563

Rabbit SDF1(Stromal Cell Derived Factor 1) ELISA Kit

Rabbit SDF1(Stromal Cell Derived Factor 1) ELISA Kit

Sensitivity

Rabbit SDF1(Stromal Cell Derived Factor 1) ELISA Kit

Overview

Product name: Rabbit SDF1(Stromal Cell Derived Factor 1) ELISA Kit
Reactivity: Rabbit
Alternative Names: CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12
Assay Type: Sandwich
Sensitivity: 23.3 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit SDF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit SDF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit SDF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit SDF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
4000.00 2.207 2.112
2000.00 1.515 1.420
1000.00 1.226 1.131
500.00 0.813 0.718
250.00 0.527 0.432
125.00 0.357 0.262
62.50 0.236 0.141
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant SDF1 and the recovery rates were calculated by comparing the measured value to the expected amount of SDF1 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 84-106% 100%
Heparin plasma(n=5) 78-95% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SDF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 87-96% 85-96% 85-95%
EDTA plasma(n=5) 87-98% 92-105% 87-98% 86-97%
Heparin plasma(n=5) 90-101% 93-101% 87-96% 78-92%
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