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ELK Biotechnology

ELK9662

Rabbit SIRT3(Sirtuin 3) ELISA Kit

Rabbit SIRT3(Sirtuin 3) ELISA Kit

Sensitivity

Rabbit SIRT3(Sirtuin 3) ELISA Kit

Overview

Product name: Rabbit SIRT3(Sirtuin 3) ELISA Kit
Reactivity: Rabbit
Alternative Names: SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit SIRT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit SIRT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit SIRT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit SIRT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 1.842 1.755
5.00 1.602 1.515
2.50 1.177 1.090
1.25 0.975 0.888
0.63 0.583 0.496
0.32 0.387 0.300
0.16 0.189 0.102
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant SIRT3 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT3 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 92-105% 98%
Heparin plasma(n=5) 96-107% 102%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 88-102% 87-102% 85-97%
EDTA plasma(n=5) 84-100% 95-103% 88-102% 91-99%
Heparin plasma(n=5) 83-95% 89-97% 85-94% 78-96%
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