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ELK Biotechnology

ELK9647

Rabbit TPS(Tryptase) ELISA Kit

Rabbit TPS(Tryptase) ELISA Kit

Sensitivity

Rabbit TPS(Tryptase) ELISA Kit

Overview

Product name: Rabbit TPS(Tryptase) ELISA Kit
Reactivity: Rabbit
Alternative Names: TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III
Assay Type: Sandwich
Sensitivity: 0.58 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Infection immunity;Rheumatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit TPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit TPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit TPS in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.132 2.036
50.00 1.663 1.567
25.00 1.217 1.121
12.50 0.796 0.700
6.25 0.593 0.497
3.13 0.357 0.261
1.57 0.235 0.139
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TPS and the recovery rates were calculated by comparing the measured value to the expected amount of TPS in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-103% 87-98% 85-96% 91-99%
EDTA plasma(n=5) 83-89% 88-104% 83-92% 96-102%
Heparin plasma(n=5) 81-93% 79-91% 89-101% 85-96%
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