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ELK Biotechnology

ELK9793

Rabbit Vaspin(Visceral Adipose Tissue Derived Serine Protease Inhibitor) ELISA Kit

Rabbit Vaspin(Visceral Adipose Tissue Derived Serine Protease Inhibitor) ELISA Kit

Sensitivity

Rabbit Vaspin(Visceral Adipose Tissue Derived Serine Protease Inhibitor) ELISA Kit

Overview

Product name: Rabbit Vaspin(Visceral Adipose Tissue Derived Serine Protease Inhibitor) ELISA Kit
Reactivity: Rabbit
Alternative Names: SERPINA12; OL-64; Visceral Adipose-Specific SERPIN; Serine Proteinase Inhibitor,Clade A Member 12; Visceral adipose-specific serpin
Assay Type: Sandwich
Sensitivity: 35.19 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Metabolic pathway;Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit Vaspin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit Vaspin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit Vaspin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit Vaspin in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
6000.00 1.946 1.850
3000.00 1.532 1.436
1500.00 1.136 1.040
750.00 0.839 0.743
375.00 0.529 0.433
187.50 0.340 0.244
93.75 0.258 0.162
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Vaspin and the recovery rates were calculated by comparing the measured value to the expected amount of Vaspin in samples.
Matrix Recovery range Average
serum(n=5) 80-97% 88%
EDTA plasma(n=5) 87-99% 92%
Heparin plasma(n=5) 84-99% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vaspin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-99% 85-96% 81-95% 95-105%
EDTA plasma(n=5) 88-102% 93-101% 90-101% 87-98%
Heparin plasma(n=5) 85-99% 98-105% 92-101% 89-105%
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