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FineTest

ER6010

Rat Bckdk ([3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial) ELISA Kit

Rat Bckdk ([3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial) ELISA Kit

size
Sensitivity
Range

Product Name

Rat Bckdk ([3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial) ELISA Kit

Alias

Bckdk ELISA Kit, [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase ELISA Kit,  mitochondrial ELISA Kit, Branched-chain alpha-ketoacid dehydrogenase kinase ELISA Kit, BCKD-kinase ELISA Kit, BCKDHKIN ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

 Bckdk  ELISA Kit allows for the in vitro quantitative determination of Bckdk concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Species

Rat

UniProt ID

Q00972

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Bckdk and the recovery rates were calculated by comparing the measured value to the expected amount of Bckdk in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

90-101

93

EDTA plasma(n=5)

87-103

96

heparin plasma(n=5)

89-105

95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bckdk and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

92-103%

89-99%

90-104%

EDTA plasma(n=5)

83-98%

84-99%

83-97%

heparin plasma(n=5)

85-89%

80-100%

80-98%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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