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Elabscience
SKU(재고 관리 코드):E-EL-R0221
Rat CD44(Cluster of Differentiation 44) ELISA Kit
Rat CD44(Cluster of Differentiation 44) ELISA Kit
The Rat CD44 (Cluster of Differentiation 44) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the quantitative detection of CD44 in rat serum, plasma, and other biological samples. This kit is specifically developed to provide researchers and clinicians with a precise and efficient method for measuring CD44 levels in rat samples, which is essential for understanding the role of CD44 in various physiological and pathological processes.
The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which involves the use of two highly specific antibodies that bind to different epitopes of CD44. The first antibody is coated onto the microplate wells, while the second antibody is conjugated with horseradish peroxidase (HRP) and serves as the detection reagent. The CD44 standard or sample is added to the wells, and the CD44 molecules are captured by the first antibody. After washing away any unbound substances, the HRP-conjugated second antibody is added, which binds to the captured CD44 molecules. The excess detection reagent is then removed, and a substrate solution is added, which reacts with the HRP enzyme to produce a colorimetric signal that is proportional to the amount of CD44 present in the sample.
The Rat CD44 ELISA Kit is highly sensitive, with a detection range of 0.156-10 ng/mL, and has a low limit of detection (LOD) of 0.078 ng/mL. The kit also has a high degree of specificity, with minimal cross-reactivity with other rat proteins. The kit components are stable and easy to use, with clear instructions provided for each step of the assay. The kit is suitable for use in a variety of research and clinical applications, including cancer research, immunology, and inflammation studies.
In summary, the Rat CD44 ELISA Kit is a reliable and accurate diagnostic tool that provides researchers and clinicians with a precise and efficient method for measuring CD44 levels in rat samples. Its high sensitivity, specificity, and ease of use make it an essential tool for understanding the role of CD44 in various physiological and pathological processes.
The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which involves the use of two highly specific antibodies that bind to different epitopes of CD44. The first antibody is coated onto the microplate wells, while the second antibody is conjugated with horseradish peroxidase (HRP) and serves as the detection reagent. The CD44 standard or sample is added to the wells, and the CD44 molecules are captured by the first antibody. After washing away any unbound substances, the HRP-conjugated second antibody is added, which binds to the captured CD44 molecules. The excess detection reagent is then removed, and a substrate solution is added, which reacts with the HRP enzyme to produce a colorimetric signal that is proportional to the amount of CD44 present in the sample.
The Rat CD44 ELISA Kit is highly sensitive, with a detection range of 0.156-10 ng/mL, and has a low limit of detection (LOD) of 0.078 ng/mL. The kit also has a high degree of specificity, with minimal cross-reactivity with other rat proteins. The kit components are stable and easy to use, with clear instructions provided for each step of the assay. The kit is suitable for use in a variety of research and clinical applications, including cancer research, immunology, and inflammation studies.
In summary, the Rat CD44 ELISA Kit is a reliable and accurate diagnostic tool that provides researchers and clinicians with a precise and efficient method for measuring CD44 levels in rat samples. Its high sensitivity, specificity, and ease of use make it an essential tool for understanding the role of CD44 in various physiological and pathological processes.
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