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ELK Biotechnology

ELK7227

Rat ChT(High Affinity Choline Transporter) ELISA Kit

Rat ChT(High Affinity Choline Transporter) ELISA Kit

Size
Sensitivity
Detection range

Rat ChT(High Affinity Choline Transporter) ELISA Kit

Overview

Product name: Rat ChT(High Affinity Choline Transporter) ELISA Kit
Reactivity: Rat
Alternative Names: SLC5A7; hCHT, CHT1; Solute Carrier Family 5 Member 7,Choline Transporter; Hemicholinium-3-sensitive choline transporter
Assay Type: Sandwich
Sensitivity: 28 pg/mL
Standard: 5000 pg/mL
Range: 78.13-5000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ChT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ChT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ChT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ChT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
5000.00 2.103 2.021
2500.00 1.628 1.546
1250.00 1.240 1.158
625.00 0.839 0.757
312.50 0.517 0.435
156.25 0.475 0.393
78.13 0.187 0.105
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ChT and the recovery rates were calculated by comparing the measured value to the expected amount of ChT in samples.
Matrix Recovery range Average
serum(n=5) 80-96% 88%
EDTA plasma(n=5) 80-92% 86%
Heparin plasma(n=5) 78-95% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ChT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 93-101% 86-97% 90-101%
EDTA plasma(n=5) 85-94% 87-101% 89-99% 85-95%
Heparin plasma(n=5) 85-94% 83-96% 87-98% 87-100%
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