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ELK Biotechnology

ELK9565

Rat GFER(Growth Factor, Augmenter Of Liver Regeneration) ELISA Kit

Rat GFER(Growth Factor, Augmenter Of Liver Regeneration) ELISA Kit

Sensitivity

Rat GFER(Growth Factor, Augmenter Of Liver Regeneration) ELISA Kit

Overview

Product name: Rat GFER(Growth Factor, Augmenter Of Liver Regeneration) ELISA Kit
Reactivity: Rat
Alternative Names: ERV1; ALR; HERV1; HPO; HPO1; HPO2; HSS; FAD-linked sulfhydryl oxidase ALR; Augmenter of liver regeneration
Assay Type: Sandwich
Sensitivity: 29 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Reproductive science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GFER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GFER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GFER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GFER in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
5000.00 2.136 2.040
2500.00 1.658 1.562
1250.00 1.175 1.079
625.00 0.871 0.775
312.50 0.457 0.361
156.25 0.336 0.240
78.13 0.238 0.142
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant GFER and the recovery rates were calculated by comparing the measured value to the expected amount of GFER in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GFER and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 82-98% 89-97% 85-93%
EDTA plasma(n=5) 87-102% 85-94% 87-98% 87-98%
Heparin plasma(n=5) 85-96% 80-96% 89-102% 87-98%
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