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Rat IGFBP-3(Insulin-like growth factor-binding protein 3) ELISA Kit
Rat IGFBP-3(Insulin-like growth factor-binding protein 3) ELISA Kit
Product Name
Rat IGFBP-3(Insulin-like growth factor-binding protein 3) ELISA Kit
Alias
IGFBP-3 ELISA Kit, IGFBP3 ELISA Kit, BP-53 ELISA Kit, IBP3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IGFBP-3 ELISA Kit allows for the in vitro quantitative determination of IGFBP-3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Rat
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IGFBP-3 and the recovery rates were calculated by comparing the measured value to the expected amount of IGFBP-3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-105 |
97 |
|
EDTA plasma(n=5) |
85-105 |
95 |
|
heparin plasma(n=5) |
85-104 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGFBP-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-105% |
86-98% |
86-103% |
|
EDTA plasma(n=5) |
85-97% |
85-99% |
83-98% |
|
heparin plasma(n=5) |
85-98% |
81-94% |
82-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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