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Elabscience
SKU(재고 관리 코드):E-EL-R0525
Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit
Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit
The RAT IKBΑ (Inhibitory Subunit of NF-ΚBΑ) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of IKBΑ in rat serum, plasma, and tissue homogenates. The kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of IKBΑ. The capture antibody is coated on the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The assay is easy to perform and provides accurate and reproducible results.
The NF-ΚB pathway is a critical signaling pathway that regulates various cellular processes, including inflammation, immune response, and cell survival. IKBΑ is a key negative regulator of the NF-ΚB pathway, which inhibits the translocation of NF-ΚB from the cytoplasm to the nucleus, where it activates the transcription of target genes. Dysregulation of the NF-ΚB pathway has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory diseases. Therefore, the measurement of IKBΑ levels in biological samples is important for understanding the regulation of the NF-ΚB pathway and its role in disease pathogenesis.
The RAT IKBΑ ELISA Kit has a detection range of 0.156-10 ng/mL and a sensitivity of 0.078 ng/mL. The kit includes all necessary reagents and a detailed protocol for performing the assay. The assay can be completed in less than 4 hours, and the results can be read using a microplate reader at 450 nm. The kit has been validated for use with rat samples, but it may also be suitable for use with other species, depending on the homology of the target protein. Overall, the RAT IKBΑ ELISA Kit is a reliable and convenient tool for the quantitative measurement of IKBΑ in rat samples, which can be used in various research applications, including drug discovery, biomarker identification, and disease diagnosis.
The NF-ΚB pathway is a critical signaling pathway that regulates various cellular processes, including inflammation, immune response, and cell survival. IKBΑ is a key negative regulator of the NF-ΚB pathway, which inhibits the translocation of NF-ΚB from the cytoplasm to the nucleus, where it activates the transcription of target genes. Dysregulation of the NF-ΚB pathway has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory diseases. Therefore, the measurement of IKBΑ levels in biological samples is important for understanding the regulation of the NF-ΚB pathway and its role in disease pathogenesis.
The RAT IKBΑ ELISA Kit has a detection range of 0.156-10 ng/mL and a sensitivity of 0.078 ng/mL. The kit includes all necessary reagents and a detailed protocol for performing the assay. The assay can be completed in less than 4 hours, and the results can be read using a microplate reader at 450 nm. The kit has been validated for use with rat samples, but it may also be suitable for use with other species, depending on the homology of the target protein. Overall, the RAT IKBΑ ELISA Kit is a reliable and convenient tool for the quantitative measurement of IKBΑ in rat samples, which can be used in various research applications, including drug discovery, biomarker identification, and disease diagnosis.
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