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Elabscience
SKU(재고 관리 코드):E-EL-R0568
Rat IL-21(Interleukin 21) ELISA Kit
Rat IL-21(Interleukin 21) ELISA Kit
The RAT IL-21 (Interleukin 21) ELISA Kit is a highly reliable and efficient tool designed for the quantitative measurement of Interleukin 21 in rat samples. Interleukin 21 is a cytokine that plays a crucial role in the regulation of immune responses, particularly in the development and function of T cells and B cells. This ELISA kit provides researchers with a precise and accurate method to assess IL-21 levels in various biological samples, enabling a deeper understanding of immune system dynamics and potential therapeutic targets.
This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. It utilizes a sandwich enzyme-linked immunosorbent assay technique, where a monoclonal antibody specific to rat IL-21 is pre-coated onto a microplate. The IL-21 present in the sample binds to the immobilized antibody, while any unbound substances are washed away. A biotin-conjugated polyclonal antibody specific to IL-21 is then added, forming a sandwich complex. This complex is further amplified by the addition of an enzyme-conjugated streptavidin, followed by the addition of a substrate solution that generates a color reaction. The intensity of the color is directly proportional to the concentration of IL-21 in the sample, which can be measured spectrophotometrically.
This ELISA kit is user-friendly and provides a wide dynamic range, allowing for the detection of IL-21 in a variety of sample types, including serum, plasma, and cell culture supernatants. It offers excellent precision with low intra-assay and inter-assay coefficients of variation, ensuring reliable and reproducible results. The kit includes all necessary components, including pre-coated microplates, antibodies, enzyme conjugates, and buffers, providing convenience and ease of use. With its high sensitivity, specificity, and accuracy, the RAT IL-21 ELISA Kit is an indispensable tool for researchers studying immune system regulation, inflammation, and potential therapeutic interventions.
This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. It utilizes a sandwich enzyme-linked immunosorbent assay technique, where a monoclonal antibody specific to rat IL-21 is pre-coated onto a microplate. The IL-21 present in the sample binds to the immobilized antibody, while any unbound substances are washed away. A biotin-conjugated polyclonal antibody specific to IL-21 is then added, forming a sandwich complex. This complex is further amplified by the addition of an enzyme-conjugated streptavidin, followed by the addition of a substrate solution that generates a color reaction. The intensity of the color is directly proportional to the concentration of IL-21 in the sample, which can be measured spectrophotometrically.
This ELISA kit is user-friendly and provides a wide dynamic range, allowing for the detection of IL-21 in a variety of sample types, including serum, plasma, and cell culture supernatants. It offers excellent precision with low intra-assay and inter-assay coefficients of variation, ensuring reliable and reproducible results. The kit includes all necessary components, including pre-coated microplates, antibodies, enzyme conjugates, and buffers, providing convenience and ease of use. With its high sensitivity, specificity, and accuracy, the RAT IL-21 ELISA Kit is an indispensable tool for researchers studying immune system regulation, inflammation, and potential therapeutic interventions.
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