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ELK Biotechnology

ELK5684

Rat IL6R(Interleukin 6 Receptor) ELISA Kit

Rat IL6R(Interleukin 6 Receptor) ELISA Kit

Size
Sensitivity
Detection range

Rat IL6R(Interleukin 6 Receptor) ELISA Kit

Overview

Product name: Rat sIL-6R(SolubleInterleukin 6 Receptor) ELISA Kit
Reactivity: Rat
Alternative Names: IL-6R;CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; Interleukin 6 Receptor
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
Standard: 10 ng/mL
Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.166 2.062
5.00 1.741 1.637
2.50 1.154 1.050
1.25 0.886 0.782
0.63 0.563 0.459
0.32 0.388 0.284
0.16 0.175 0.071
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sIL-6R and the recovery rates were calculated by comparing the measured value to the expected amount of sIL-6R in samples.
Matrix Recovery range Average
serum(n=5) 80-93% 86%
EDTA plasma(n=5) 94-105% 98%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sIL-6R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-105% 82-101% 91-103% 95-102%
EDTA plasma(n=5) 87-91% 85-93% 89-97% 85-93%
Heparin plasma(n=5) 89-97% 85-94% 87-96% 85-97%
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