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ELK Biotechnology

ELK9634

Rat VAP-1(VascularAdhesion Protein 1) ELISA Kit

Rat VAP-1(VascularAdhesion Protein 1) ELISA Kit

Sensitivity

Rat VAP-1(VascularAdhesion Protein 1) ELISA Kit

Overview

Product name: Rat VAP-1(VascularAdhesion Protein 1) ELISA Kit
Reactivity: Rat
Alternative Names: AOC3 ; HPAO; SSAO; Amine oxidase; copper containing 3
Assay Type: Sandwich
Sensitivity: 0.10 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Neuroscience;Signal Transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VAP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VAP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VAP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VAP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.096 2.008
5.00 1.663 1.575
2.50 1.115 1.027
1.25 0.831 0.743
0.63 0.655 0.567
0.32 0.354 0.266
0.16 0.175 0.087
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant VAP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of VAP-1 in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 89-103% 96%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VAP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 97-105% 84-96% 86-97%
EDTA plasma(n=5) 87-102% 89-101% 89-105% 90-101%
Heparin plasma(n=5) 85-97% 82-93% 95-102% 89-99%
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