RBBP5; RBQ3; Retinoblastoma-binding protein 5; RBBP-5; Retinoblastoma-binding protein RBQ-3
Applications:
WB;IHC
Recommended Dilutions:
WB 1:500-2000;IHC-p 1:50-300
Immunogen:
Synthesized peptide derived from RBQ-3 . at AA range: 190-270
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
59kD
GeneID（Human）:
5929
Human Swiss-Prot No:
Q15291
Cellular localization:
Nucleus .
Background:
This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2010],
Western Blot analysis of CoLo cells using RBQ-3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).