SKU(재고 관리 코드):RPB908Ra01
Recombinant Aggrecan (AGC)
Recombinant Aggrecan (AGC)
Prokaryotic expression
Product No.
RPB908Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted
Predicted Molecular Mass
13.8kDa
Accurate Molecular Mass
17kDa(Analysis of differences refer to the manual)
Residues & Tags
Pro34~Glu147 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 95%
Isoelectric Point
6.0
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Magazine | Citations |
Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source |
Age( dordr). | In vitro tenocyte metabolism in aging and oestrogen deficiency Springer: Source |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source |
Stem Cell Research & Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. Pubmed: 24739658 |
Materials Science and Engineering: C | Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition ScienceDirect: S0928493113006656 |
PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592 |
Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747 |
Macromolecular Bioscience | Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration PubMed: 25787871 |
Osteoarthritis and Cartilage | Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs Pubmed:26802547 |
Cell Proliferation | Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes Pubmed:27090063 |
bmc musculoskeletal disorders | Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. pubmed:28187731 |
Journal of Clinical Medicine | Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors Pubmed: 32392807 |
Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs | |
researchsquare | Aggrecan-a new biomarker for acute type A aortic dissection 33990642 |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
RPB908Ra01 | Recombinant Aggrecan (AGC) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB908Ra02 | Recombinant Aggrecan (AGC) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB908Ra02 | Polyclonal Antibody to Aggrecan (AGC) | WB; IHC; ICC; IP. |
PAB908Ra01 | Polyclonal Antibody to Aggrecan (AGC) | WB; IHC; ICC; IP. |
LAB908Ra71 | Biotin-Linked Polyclonal Antibody to Aggrecan (AGC) | WB; IHC; ICC. |
SEB908Ra | ELISA Kit for Aggrecan (AGC) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB908Ra | Magnetic Luminex Assay Kit for Aggrecan (AGC) ,etc. | Magnetic Luminex Assay for Antigen Detection. |