Recombinant Chymotrypsin C (CTRC)

Prokaryotic expression

Product No.

RPB310Hu01

Organism Species

Homo sapiens (Human).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Secreted

Predicted Molecular Mass

56.6kDa

Accurate Molecular Mass

57kDa(Analysis of differences refer to the manual)

Residues & Tags

Val30~Leu268 with N-terminal His and GST Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

6.6

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

20µl 100µl 200µl 1ml 10ml

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.