Recombinant Human GLS

AAD20; EC 3.5.1.2; GLS; GLS1DKFZp686O15119; glutaminase C; glutaminase kidney isoform, mitochondrial; Glutaminase; glutaminase, phosphate-activated; K-Glutaminase; KIAA0838FLJ10358; L-Glutamine Amidohydrolase

Product Name

Recombinant Human GLS

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

609-669

Mol. Weight

27.7 KDa

Tags

N-terminal His-IF2DI Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

AAD20, EC 3.5.1.2, GLS, GLS1DKFZp686O15119, glutaminase C, glutaminase kidney isoform, mitochondrial, Glutaminase, glutaminase, phosphate-activated, K-Glutaminase, KIAA0838FLJ10358, L-Glutamine Amidohydrolase