Recombinant Human MCP3

Product Name

Recombinant Human MCP3

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

24-99

Mol. Weight

8.3 kDa

Tags

N-terminal His Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 0.1-0.5 µg/mL

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

C-C motif chemokine 7, Ccl7, CCL7_HUMAN, Chemokine CC motif ligand 7, FIC, MARC, MCP-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, RP23-350G1.4, SCYA6, SCYA7, Small-inducible cytokine A7