Recombinant Human TGF beta 1

Product Name

Recombinant Human TGFB1

Size

50μg, 200μg, 1mg

Species

Human

Host

HEK293 cells

Expression Region

30-390

Mol. Weight

16,38,55 kDa

Tags

C-terminal His Tag

Purity

Greater than 90% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.05-0.3 ng/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

CED, DPD1, LAP, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, TGF-beta 1, tgfbeta1, tgf-beta1, TGFβ, TGF-β, TGFβ1, TGF-β1