Recombinant Leukemia Inhibitory Factor (LIF)

Prokaryotic expression

Product No.

RPA085Mu01

Organism Species

Mus musculus (Mouse).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1μg

Subcellular Location

Secreted

Predicted Molecular Mass

21.3kDa

Accurate Molecular Mass

21kDa(Analysis of differences refer to the manual)

Residues & Tags

Pro25~Phe203 with N-terminal His Tag

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Freeze-dried powder

Purity

> 90%

Isoelectric Point

9.1

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine	Citations
British Journal of Anaesthesia	Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioids pubmed:28077540
Biological Rhythm Research	A study of altered cytokine rhythms associated with successful implantation in cows 10.1080/09291016.2017.1361159
	Integrated analysis of cancer stem cells-associated lncRNA-miRNA-mRNA network for ovarian cancer via microarray and Gene Expression Omnibus database

Catalog No.	Related products for research use of Mus musculus (Mouse) Organism species	Applications (RESEARCH USE ONLY!)
APA085Mu61	Active Leukemia Inhibitory Factor (LIF)	Cell culture; Activity Assays.
APA085Mu01	Active Leukemia Inhibitory Factor (LIF)	Cell culture; Activity Assays.
RPA085Mu01	Recombinant Leukemia Inhibitory Factor (LIF)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA085Mu01	Polyclonal Antibody to Leukemia Inhibitory Factor (LIF)	WB; IHC; ICC; IP.
SEA085Mu	ELISA Kit for Leukemia Inhibitory Factor (LIF)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA085Mu	Magnetic Luminex Assay Kit for Leukemia Inhibitory Factor (LIF) ,etc.	Magnetic Luminex Assay for Antigen Detection.