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Cloud-clone

SKU(재고 관리 코드):RPC302Hu01

Recombinant N-Acylsphingosine Amidohydrolase 1 (ASAH1)

Recombinant N-Acylsphingosine Amidohydrolase 1 (ASAH1)

UOM
Purity
Organism species

Prokaryotic expression

Product No.

RPC302Hu01

Organism Species

Homo sapiens (Human).

Source

Prokaryotic expression

Host

E.coli

Endotoxin Level

<1.0EU per 1µg (determined by the LAL method)

Subcellular Location

Lysosome

Predicted Molecular Mass

32.7kDa

Accurate Molecular Mass

33kDa(Analysis of differences refer to the manual)

Residues & Tags

Thr144~Trp395 with N-terminal His Tag

Buffer Formulation

20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Traits

Freeze-dried powder

Purity

> 97%

Isoelectric Point

7.5

Applications

Positive Control; Immunogen; SDS-PAGE; WB.

If bio-activity of the protein is needed, please check active protein.

UOM

10µg 50µg 200µg 1mg 5mg

SEQUENCE

 

 

 

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Cytotechnology The investigation of ceranib-2 on apoptosis and drug interaction with carboplatin in human non small cell lung cancer cells in vitro pubmed:29230631
Cells Targeting Acid Ceramidase to Improve the Radiosensitivity of Rectal Cancer 33334013
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC302Hu01 Recombinant N-Acylsphingosine Amidohydrolase 1 (ASAH1) Positive Control; Immunogen; SDS-PAGE; WB.
PAC302Hu01 Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 1 (ASAH1) WB; IHC; ICC; IP.
MAC302Hu22 Monoclonal Antibody to N-Acylsphingosine Amidohydrolase 1 (ASAH1) WB; IHC; ICC; IP.
SEC302Hu ELISA Kit for N-Acylsphingosine Amidohydrolase 1 (ASAH1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC302Hu Magnetic Luminex Assay Kit for N-Acylsphingosine Amidohydrolase 1 (ASAH1) ,etc. Magnetic Luminex Assay for Antigen Detection.
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