Cloud-clone
SKU(재고 관리 코드):RPB622Ra01
Recombinant Surfactant Protein B (SP-B)
Recombinant Surfactant Protein B (SP-B)
Prokaryotic expression
Product No.
RPB622Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted, Extracellular matrix
Predicted Molecular Mass
28.3kDa
Accurate Molecular Mass
26kDa(Analysis of differences refer to the manual)
Residues & Tags
Arg111~His329 with N-terminal His Tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
8.3
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Respiratory Physiology & Neurobiology | Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgery PubMed: 21736957 |
| American Journal of Rhinology & Allergy | Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps PubMed: 23406594 |
| 19 | Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot study PubMed: 20650982 |
| Plos one | Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins PubMed: PMC3551896 |
| Plos one | The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS PubMed: PMC3787032 |
| Cancer Cell. | Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models PubMed: PMC3406925 |
| Respir Physiol Neurobiol | Acute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps project Pubmed: 23619193 |
| PLOS ONE | Surfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart Failure Pubmed:25514679 |
| Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge Opus4:Source | |
| Int J Cardiol | Plasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective study PubMed: 26310985 |
| Plos One | Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) Pubmed:27337142 |
| PLoS One | The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions. pubmed:27656877 |
| Frontiers in Aging Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid. pubmed:28101052 |
| Frontiers in Aging Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid PMC5209370 |
| Respiratory Physiology & Neurobiology | Diving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional divers pubmed:28467885 |
| Cellular Physiology and Biochemistry | Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production pubmed:28743125 |
| American Journal of Physiology. Lung Cellular and Molecular Physiology | Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. pubmed:29167126 |
| Respiratory research | Effects and molecular mechanisms of intrauterine infection/inflammation on lung development Pubmed:29747649 |
| Respiratory Physiology & Neurobiology | Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan Pubmed:29548887 |
| Lung | Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation Pubmed:29445934 |
| American Journal of Physiology Lung Cellular andMolecular Physiology | Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells Pubmed: 30211653 |
| Respiratory Research | Culture of human alveolar epithelial type II cells by sprouting Pubmed: 30340591 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB622Ra01 | Recombinant Surfactant Protein B (SP-B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB622Ra01 | Polyclonal Antibody to Surfactant Protein B (SP-B) | WB; IHC; ICC; IP. |
| MAB622Ra21 | Monoclonal Antibody to Surfactant Protein B (SP-B) | WB; IHC; ICC; IP. |
| SEB622Ra | ELISA Kit for Surfactant Protein B (SP-B) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB622Ra | Magnetic Luminex Assay Kit for Surfactant Protein B (SP-B) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB622Ra01 | ELISA Kit DIY Materials for Surfactant Protein B (SP-B) |
Main materials for "Do It (ELISA Kit) Yourself". |
