Cloud-clone
SKU(재고 관리 코드):RPP549Ra01
Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
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Prokaryotic expression
Product No.
RPP549Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Prokaryotic expression
Host
E.coli
Endotoxin Level
<1.0EU per 1µg (determined by the LAL method)
Subcellular Location
Secreted, Extracellular matrix
Predicted Molecular Mass
39.5kDa
Accurate Molecular Mass
40kDa(Analysis of differences refer to the manual)
Residues & Tags
Ile62~Lys380 with N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits
Freeze-dried powder
Purity
> 97%
Isoelectric Point
8.8
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
UOM
10µg 50µg 200µg 1mg 5mg
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Liver International | Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis Pubmed:25488180 |
| Journal of Physiology and Biochemistry | Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells PubMed: 26547443 |
| Liver | Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis PubMed: 25488180 |
| Transl Res | Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures PubMed: 26163991 |
| 不确定杂志名 | Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an … servlets:MCRFileNodeServlet |
| Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. pubmed:28754618 |
| Arteriosclerosis, Thrombosis, and Vascular Biology | Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With … Pubmed:29674475 |
| Stem Cells | Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells Pubmed:29377497 |
| Science Translational Medicine | Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidases Pubmed: 31534019 |
| J Clin Endocrinol Metab | Role of wnt5a in Metabolic Inflammation in Humans Pubmed: 30137542 |
| Cancer Research | Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis Pubmed: 31932454 |
| CLINICAL BIOCHEMISTRY | Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease Pubmed: 33188773 |
| Sao Paulo Medical Journal | Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … Pubmed: 32491086 |
| Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties 34823885 |
| PLoS One | Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study Pubmed:34990470 |
| J Clin Periodontol | Association between bone turnover markers and periodontitis: A population‐based cross‐sectional study Pubmed:35569034 |
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