RPL10A; NEDD6; 60S ribosomal protein L10a; CSA-19; Neural precursor cell expressed developmentally down-regulated protein 6; NEDD-6
Applications:
WB;IHC
Recommended Dilutions:
WB 1:500-2000;IHC-p 1:50-300
Immunogen:
Synthesized peptide derived from Ribosomal Protein L10A . at AA range: 30-110
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
25kD
GeneID（Human）:
4736
Human Swiss-Prot No:
P62906
Cellular localization:
nucleus,nucleolus,cytoplasm,cytosol,ribosome,focal adhesion,large ribosomal subunit,membrane,cytosolic large ribosomal subunit,extracellular exosome,
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes
Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°over night
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).