RNF138; NARF; HSD-4; HSD4; E3 ubiquitin-protein ligase RNF138; Nemo-like kinase-associated RING finger protein; NLK-associated RING finger protein; hNARF; RING finger protein 138
Applications:
WB;IHC
Recommended Dilutions:
WB 1:500-2000;IHC-p 1:50-300
Immunogen:
The antiserum was produced against synthesized peptide derived from human RNF138. AA range:31-80
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
28kD
GeneID（Human）:
51444
Human Swiss-Prot No:
Q8WVD3
Cellular localization:
Chromosome . Recruited at DNA damage sites (PubMed:26502055). Localizes to sites of double-strand break: localization to double-strand break sites is mediated by the zinc fingers (PubMed:26502055, PubMed:26502057). .
Background:
ring finger protein 138(RNF138) Homo sapiens The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
Western blot analysis of lysates from MCF-7 cells, using RNF138 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).