1 1

ELK Biotechnology

ELK9627

Simian OC/BGP(Osteocalcin) ELISA Kit

Simian OC/BGP(Osteocalcin) ELISA Kit

Sensitivity

Simian OC/BGP(Osteocalcin) ELISA Kit

Overview

Product name: Simian OC/BGP(Osteocalcin) ELISA Kit
Reactivity: Simian
Alternative Names: BGLAP; BGP; OC; OCN; bone gamma-carboxyglutamate protein; Osteocalcin
Assay Type: Sandwich
Sensitivity: 0.47 ng/mL
Standard: 50 ng/mL
Detection Range: 0.78-50 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Stem Cells;Signal Transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian OC/BGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian OC/BGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian OC/BGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian OC/BGP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 2.266 2.171
25.00 1.593 1.498
12.50 1.228 1.133
6.25 0.757 0.662
3.13 0.563 0.468
1.57 0.318 0.223
0.79 0.236 0.141
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant OC/BGP and the recovery rates were calculated by comparing the measured value to the expected amount of OC/BGP in samples.
Matrix Recovery range Average
serum(n=5) 78-96% 87%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 90-103% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OC/BGP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 87-103% 81-93% 79-92%
EDTA plasma(n=5) 89-102% 87-98% 80-92% 98-105%
Heparin plasma(n=5) 84-99% 87-96% 88-97% 89-102%
전체 세부 정보 보기