Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human SPHK2. AA range:580-629
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
70kD
GeneID（Human）:
56848
Human Swiss-Prot No:
Q9NRA0
Cellular localization:
Cytoplasm . Nucleus . Endoplasmic reticulum . Mitochondrion inner membrane . In nucleus, located in nucleosomes where it associates with core histone proteins such as histone 3 (PubMed:19729656). In brains of patients with Alzheimer's disease, may be preferentially localized in the nucleus. Cytosolic expression decrease correlates with the density of amyloid deposits (PubMed:29615132). In apoptotic cells, colocalizes with CASP1 in cell membrane where is cleaved and released from cells in an active form (PubMed:20197547). .; [Isoform 2]: Lysosome membrane .
Background:
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011],
Western Blot analysis of 22RV1 cells using SphK2 Polyclonal Antibody diluted at 1:500
Western Blot analysis of AD293 using SphK2 Polyclonal Antibody diluted at 1:500
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SPHK2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, using SPHK2 Antibody. The lane on the right is blocked with the synthesized peptide.